CIIT Flow Cytometry Core
Flow Cytometry Services
The Flow Cytometry Core supports faculty and students at Seattle Children's Research Institute as well as investigators at other academic institutions and private companies.
The core provides access to state-of-the-art cell sorters, analyzers and workstations; training and technical support of investigators; assistance with experimental design, data analysis and interpretation; and assistance in developing novel multicolor flow cytometric techniques.
In addition to reading about the instruments available in our lab, you can view links to helpful information about reagents and cytometry tools that may be helpful as you set up and run your experiments.
The core has two BD LSRII instruments. Each of these instruments has five lasers, and each LSR has 18 fluorescent parameters in addition to the FSC and SSC parameters. The lasers are 355nm, 405nm, 488nm, 561nm, and 635nm. Both LSR instruments also have a high throughput system (HTS) that acquires samples from multi-well plates.
The core has a BD ARIA sorter that has five lasers, and 18 fluorescent parameters in addition to the FSC and SSC parameters. The lasers are 355nm, 405nm, 488nm, 561nm, and 635nm. This ARIA matches the lasers, parameters and filters of both LSRs.
The core also has a BD ARIA sorter that has three lasers, and 9 fluorescent parameters in addition to the FSC and SSC parameters. The lasers are 405nm, 488nm, and 635nm.
Both ARIA instruments can perform up to four-way bulk sorting for a variety of tube sizes. Both instruments can also sort into multi-well plates. Both ARIA instruments are equipped with an aerosol management option (AMO).
Below are links to information on reagents that may be helpful as you set up and run your experiments:
Below are links to information on cytometry tools that may be helpful as you set up and run your experiments:
View contact information for the Flow Cytometry Core.
For More Information
- See Applications of Flow Cytometry for information on immunophenotyping, cell sorting, cell cycle analysis, apoptosis, cell proliferation assays and intracellular calcium flux.
- See Flow Cytometry Fee Structure for details on fees.
- See How to Schedule Instrument Use for scheduling details.
- Frequently Asked Questions