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Cheng RY, Hung KL, Zhang T, Stoffers CM, Ott AR, Suchland ER, Camp ND, Khan IF, Singh S, Yang YJ, Rawlings DJ, James RG.
Ex vivo engineered human plasma cells exhibit robust protein secretion and long-term engraftment in vivo.
Nat Commun. 2022 Oct 16;13(1):6110. doi: 10.1038/s41467-022-33787-8. PMID: 36245034; PMCID: PMC9573882.

Muir V, Sagadiev S, Liu S, Holder U, Armendariz AM, Suchland E, Meitlis I, Camp N, Giltiay N, Tam JM, Garner EC, Wivagg CN, Shows D, James RG, Lacy-Hulbert A, Acharya M.
Transcriptomic analysis of pathways associated with ITGAV/alpha(v) integrin-dependent autophagy in human B cells.
Autophagy. 2022 Aug 25:1-17. doi: 10.1080/15548627.2022.2113296. Epub ahead of print. PMID: 36016494.

Vamva E, Ozog S, Verhoeyen E, James RG, Rawlings DJ, Torbett BE.
An optimized measles virus glycoprotein-pseudotyped lentiviral vector production system to promote efficient transduction of human primary B cells.
STAR Protoc. 2022 Mar 8;3(1):101228. doi: 10.1016/j.xpro.2022.101228. PMID: 35284833; PMCID: PMC8914380.

Al Qureshah F, Sagadiev S, Thouvenel CD, Liu S, Hua Z, Hou B, Acharya M, James RG, Rawlings DJ.
Activated PI3Kδ signals compromise plasma cell survival via limiting autophagy and increasing ER stress.
J Exp Med. 2021 Dec 6;218(12). doi: 10.1084/jem.20211035. Epub 2021 Sep 29. PubMed PMID: 34586341.

Saha SS, Samanas NB, Miralda I, Shubin NJ, Niino K, Bhise G, Acharya M, Seo AJ, Camp N, Deutsch GH, James RG, Piliponsky AM.
Mast cell surfaceome characterization reveals CD98 heavy chain is critical for optimal cell function.
J Allergy Clin Immunol. 2021 Jul 27;. doi: 10.1016/j.jaci.2021.07.014. [Epub ahead of print] PubMed PMID: 34324892.

Thomas KR, Allenspach EJ, Camp ND, Wray-Dutra MN, Khim S, Zielinska-Kwiatkowska A, Timms AE, Loftus JP, Liggitt HD, Georgopoulos K, Tasian SK, James RG, Rawlings DJ.
Activated interleukin-7 receptor signaling drives B-cell acute lymphoblastic leukemia in mice.
Leukemia. 2021 Jun 30;. doi: 10.1038/s41375-021-01326-x. [Epub ahead of print] PubMed PMID: 34193976.

Meitlis I, Allenspach EJ, Bauman BM, Phan IQ, Dabbah G, Schmitt EG, Camp ND, Torgerson TR, Nickerson DA, Bamshad MJ, Hagin D, Luthers CR, Stinson JR, Gray J, Lundgren I, Church JA, Butte MJ, Jordan MB, Aceves SS, Schwartz DM, Milner JD, Schuval S, Skoda-Smith S, Cooper MA, Starita LM, Rawlings DJ, Snow AL, James RG.
Multiplexed Functional Assessment of Genetic Variants in CARD11.
Am J Hum Genet. 2020 Dec 3;107(6):1029-1043. doi: 10.1016/j.ajhg.2020.10.015. Epub 2020 Nov 16. PubMed PMID: 33202260; PubMed Central PMCID: PMC7820631.

Burleigh K, Maltbaek JH, Cambier S, Green R, Gale M Jr, James RC, Stetson DB.
Human DNA-PK activates a STING-independent DNA sensing pathway.
Sci Immunol. 2020 Jan 24;5(43). doi: 10.1126/sciimmunol.aba4219. PubMed PMID: 31980485; PubMed Central PMCID: PMC7081723.

Gelman H, Dines JN, Berg J, Berger AH, Brnich S, Hisama FM, James RG, Rubin AF, Shendure J, Shirts B, Fowler DM, Starita LM.
Recommendations for the collection and use of multiplexed functional data for clinical variant interpretation.
Genome Med. 2019 Dec 20;11(1):85. doi: 10.1186/s13073-019-0698-7. PubMed PMID: 31862013; PubMed Central PMCID: PMC6925490.

Ramírez JA, Iwata T, Park H, Tsang M, Kang J, Cui K, Kwong W, James RG, Baba M, Schmidt LS, Iritani BM.
Folliculin Interacting Protein 1 Maintains Metabolic Homeostasis during B Cell Development by Modulating AMPK, mTORC1, and TFE3.
J Immunol. 2019 Dec 1;203(11):2899-2908. doi: 10.4049/jimmunol.1900395. Epub 2019 Nov 1. PubMed PMID: 31676673; PubMed Central PMCID: PMC6864314.

Carpentier SJ, Ni M, Duggan JM, James RG, Cookson BT, Hamerman JA.
The signaling adaptor BCAP inhibits NLRP3 and NLRC4 inflammasome activation in macrophages through interactions with Flightless-1.
Sci Signal. 2019 May 14;12(581). doi: 10.1126/scisignal.aau0615. PubMed PMID: 31088976; PubMed Central PMCID: PMC6604799.

Wray-Dutra MN, Al Qureshah F, Metzler G, Oukka M, James RG, Rawlings DJ.
Activated PIK3CD drives innate B cell expansion yet limits B cell-intrinsic immune responses.
J Exp Med. 2018 Oct 1;215(10):2485-2496. doi: 10.1084/jem.20180617. Epub 2018 Sep 7. PubMed PMID: 30194267; PubMed Central PMCID: PMC6170176.

Wray-Dutra MN, Chawla R, Thomas KR, Seymour BJ, Arkatkar T, Sommer KM, Khim S, Trapnell C, James RG, Rawlings DJ.
Activated CARD11 accelerates germinal center kinetics, promoting mTORC1 and terminal differentiation.
J Exp Med. 2018 Sep 3;215(9):2445-2461. doi: 10.1084/jem.20180230. Epub 2018 Aug 20. PubMed PMID: 30127060; PubMed Central PMCID: PMC6122963.

Altman MC, Reeves SR, Parker AR, Whalen E, Misura KM, Barrow KA, James RG, Hallstrand TS, Ziegler SF, Debley JS.
Interferon response to respiratory syncytial virus by bronchial epithelium from children with asthma is inversely correlated with pulmonary function.
J Allergy Clin Immunol. 2018 Aug;142(2):451-459. doi: 10.1016/j.jaci.2017.10.004. Epub 2017 Oct 26. PubMed PMID: 29106997; PubMed Central PMCID: PMC5951761.

James RG, Reeves SR, Barrow KA, White MP, Glukhova VA, Haghighi C, Seyoum D, Debley JS.
Deficient Follistatin-like 3 Secretion by Asthmatic Airway Epithelium Impairs Fibroblast Regulation and Fibroblast-to-Myofibroblast Transition.
Am J Respir Cell Mol Biol. 2018 Jul;59(1):104-113. doi: 10.1165/rcmb.2017-0025OC. PubMed PMID: 29394092; PubMed Central PMCID: PMC6039878.

Hung KL, Meitlis I, Hale M, Chen CY, Singh S, Jackson SW, Miao CH, Khan IF, Rawlings DJ, James RG.
Engineering Protein-Secreting Plasma Cells by Homology-Directed Repair in Primary Human B Cells.
Mol Ther. 2018 Feb 7;26(2):456-467. doi: 10.1016/j.ymthe.2017.11.012. Epub 2017 Nov 22. PubMed PMID: 29273498; PubMed Central PMCID: PMC5835153.

Gorman JA, Hundhausen C, Errett JS, Stone AE, Allenspach EJ, Ge Y, Arkatkar T, Clough C, Dai X, Khim S, Pestal K, Liggitt D, Cerosaletti K, Stetson DB, James RG, Oukka M, Concannon P, Gale M Jr, Buckner JH, Rawlings DJ.
The A946T variant of the RNA sensor IFIH1 mediates an interferon program that limits viral infection but increases the risk for autoimmunity.
Nat Immunol. 2017 Jul;18(7):744-752. doi: 10.1038/ni.3766. Epub 2017 May 29. PubMed PMID: 28553952; PubMed Central PMCID: PMC5697900.

Shubin NJ, Glukhova VA, Clauson M, Truong P, Abrink M, Pejler G, White NJ, Deutsch GH, Reeves SR, Vaisar T, James RG, Piliponsky AM.
Proteome analysis of mast cell releasates reveals a role for chymase in the regulation of coagulation factor XIIIA levels via proteolytic degradation.
J Allergy Clin Immunol. 2017 Jan;139(1):323-334. doi: 10.1016/j.jaci.2016.03.051. Epub 2016 May 13. PubMed PMID: 27302551; PubMed Central PMCID: PMC5107356.

Dam EM, Habib T, Chen J, Funk A, Glukhova V, Davis-Pickett M, Wei S, James R, Buckner JH, Cerosaletti K.
The BANK1 SLE-risk variants are associated with alterations in peripheral B cell signaling and development in humans.
Clin Immunol. 2016 Dec;173:171-180. doi: 10.1016/j.clim.2016.10.018. Epub 2016 Nov 2. PubMed PMID: 27816669; PubMed Central PMCID: PMC5148640.

Tampella G, Kerns HM, Niu D, Singh S, Khim S, Bosch KA, Garrett ME, Moguche A, Evans E, Browning B, Jahan TA, Nacht M, Wolf-Yadlin A, Plebani A, Hamerman JA, Rawlings DJ, James RG.
The Tec Kinase-Regulated Phosphoproteome Reveals a Mechanism for the Regulation of Inhibitory Signals in Murine Macrophages.
J Immunol. 2015 Jul 1;195(1):246-56. doi: 10.4049/jimmunol.1403238. Epub 2015 May 29. PubMed PMID: 26026062; PubMed Central PMCID: PMC4475445.

Robin NC, Agoston Z, Biechele TL, James RG, Berndt JD, Moon RT.
Simvastatin promotes adult hippocampal neurogenesis by enhancing Wnt/β-catenin signaling.
Stem Cell Reports. 2014 Jan 14;2(1):9-17. doi: 10.1016/j.stemcr.2013.11.002. eCollection 2014 Jan 14. PubMed PMID: 24511465; PubMed Central PMCID: PMC3916759.

James RG, Bosch KA, Kulikauskas RM, Yang PT, Robin NC, Toroni RA, Biechele TL, Berndt JD, von Haller PD, Eng JK, Wolf-Yadlin A, Chien AJ, Moon RT.
Protein kinase PKN1 represses Wnt/β-catenin signaling in human melanoma cells.
J Biol Chem. 2013 Nov 29;288(48):34658-70. doi: 10.1074/jbc.M113.500314. Epub 2013 Oct 10. PubMed PMID: 24114839; PubMed Central PMCID: PMC3843078.

Moore KE, Carlson SM, Camp ND, Cheung P, James RG, Chua KF, Wolf-Yadlin A, Gozani O.
A general molecular affinity strategy for global detection and proteomic analysis of lysine methylation.
Mol Cell. 2013 May 9;50(3):444-56. doi: 10.1016/j.molcel.2013.03.005. Epub 2013 Apr 11. PubMed PMID: 23583077; PubMed Central PMCID: PMC3660009.

Dai X, James RG, Habib T, Singh S, Jackson S, Khim S, Moon RT, Liggitt D, Wolf-Yadlin A, Buckner JH, Rawlings DJ.
A disease-associated PTPN22 variant promotes systemic autoimmunity in murine models.
J Clin Invest. 2013 May;123(5):2024-36. doi: 10.1172/JCI66963. Epub 2013 Apr 24. PubMed PMID: 23619366; PubMed Central PMCID: PMC3638909.

Fleming BM, Yelin R, James RG, Schultheiss TM.
A role for Vg1/Nodal signaling in specification of the intermediate mesoderm.
Development. 2013 Apr;140(8):1819-29. doi: 10.1242/dev.093740. PubMed PMID: 23533180; PubMed Central PMCID: PMC3621495.

James RG, Davidson KC, Bosch KA, Biechele TL, Robin NC, Taylor RJ, Major MB, Camp ND, Fowler K, Martins TJ, Moon RT.
WIKI4, a novel inhibitor of tankyrase and Wnt/ß-catenin signaling.
PLoS One. 2012;7(12):e50457. doi: 10.1371/journal.pone.0050457. Epub 2012 Dec 5. PubMed PMID: 23227175; PubMed Central PMCID: PMC3515623.

Silvério KG, Davidson KC, James RG, Adams AM, Foster BL, Nociti FH Jr, Somerman MJ, Moon RT.
Wnt/β-catenin pathway regulates bone morphogenetic protein (BMP2)-mediated differentiation of dental follicle cells.
J Periodontal Res. 2012 Jun;47(3):309-19. doi: 10.1111/j.1600-0765.2011.01433.x. Epub 2011 Dec 11. PubMed PMID: 22150562; PubMed Central PMCID: PMC3865600.

Camp ND, James RG, Dawson DW, Yan F, Davison JM, Houck SA, Tang X, Zheng N, Major MB, Moon RT.
Wilms tumor gene on X chromosome (WTX) inhibits degradation of NRF2 protein through competitive binding to KEAP1 protein.
J Biol Chem. 2012 Feb 24;287(9):6539-50. doi: 10.1074/jbc.M111.316471. Epub 2012 Jan 3. PubMed PMID: 22215675; PubMed Central PMCID: PMC3307315.

Biechele TL, Kulikauskas RM, Toroni RA, Lucero OM, Swift RD, James RG, Robin NC, Dawson DW, Moon RT, Chien AJ.
Wnt/β-catenin signaling and AXIN1 regulate apoptosis triggered by inhibition of the mutant kinase BRAFV600E in human melanoma.
Sci Signal. 2012 Jan 10;5(206):ra3. doi: 10.1126/scisignal.2002274. PubMed PMID: 22234612; PubMed Central PMCID: PMC3297477.

Kamei CN, Kempf H, Yelin R, Daoud G, James RG, Lassar AB, Tabin CJ, Schultheiss TM.
Promotion of avian endothelial cell differentiation by GATA transcription factors.
Dev Biol. 2011 May 1;353(1):29-37. doi: 10.1016/j.ydbio.2011.02.016. Epub 2011 Feb 23. PubMed PMID: 21354132; PubMed Central PMCID: PMC3165022.

Cimetta E, Cannizzaro C, James R, Biechele T, Moon RT, Elvassore N, Vunjak-Novakovic G.
Microfluidic device generating stable concentration gradients for long term cell culture: application to Wnt3a regulation of β-catenin signaling.
Lab Chip. 2010 Dec 7;10(23):3277-83. doi: 10.1039/c0lc00033g. Epub 2010 Oct 11. PubMed PMID: 20936235; PubMed Central PMCID: PMC4106280.

James RG, Biechele TL, Conrad WH, Camp ND, Fass DM, Major MB, Sommer K, Yi X, Roberts BS, Cleary MA, Arthur WT, MacCoss M, Rawlings DJ, Haggarty SJ, Moon RT.
Bruton's tyrosine kinase revealed as a negative regulator of Wnt-beta-catenin signaling.
Sci Signal. 2009 May 26;2(72):ra25. doi: 10.1126/scisignal.2000230. PubMed PMID: 19471023; PubMed Central PMCID: PMC4790753.

James RG, Conrad WH, Moon RT.
Beta-catenin-independent Wnt pathways: signals, core proteins, and effectors.
Methods Mol Biol. 2008;468:131-44. doi: 10.1007/978-1-59745-249-6_10. PubMed PMID: 19099251.

James RG, Kamei CN, Wang Q, Jiang R, Schultheiss TM.
Odd-skipped related 1 is required for development of the metanephric kidney and regulates formation and differentiation of kidney precursor cells.
Development. 2006 Aug;133(15):2995-3004. doi: 10.1242/dev.02442. Epub 2006 Jun 21. PubMed PMID: 16790474.

James RG, Schultheiss TM.
Bmp signaling promotes intermediate mesoderm gene expression in a dose-dependent, cell-autonomous and translation-dependent manner.
Dev Biol. 2005 Dec 1;288(1):113-25. doi: 10.1016/j.ydbio.2005.09.025. Epub 2005 Oct 21. PubMed PMID: 16243309.

Wilm B, James RG, Schultheiss TM, Hogan BL.
The forkhead genes, Foxc1 and Foxc2, regulate paraxial versus intermediate mesoderm cell fate.
Dev Biol. 2004 Jul 1;271(1):176-89. doi: 10.1016/j.ydbio.2004.03.034. PubMed PMID: 15196959.

James RG, Schultheiss TM.
Patterning of the avian intermediate mesoderm by lateral plate and axial tissues.
Dev Biol. 2003 Jan 1;253(1):109-24. doi: 10.1006/dbio.2002.0863. PubMed PMID: 12490201.


Richard James

For questions or inquiries, 206. 884. 3290


James Lab
1900 9th Avenue
Seattle, WA 98101

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