Kenneth D. Stuart, PhD

Infectious Disease Research

Children's Title: Professor

Academic Title: Affiliate Professor, University of Washington

Research Center: Center for Global Infectious Disease Research

  • Ken Stuart, PhD, is a professor in the Center of Global Infectious Disease Research at Seattle Children’s Research Institute, an affiliate professor of Global Health at the University of Washington where he chaired the Department of Pathobiology (1996 to 2004) and an affiliate investigator in the Vaccine and Infectious Disease Division at the Fred Hutchinson Research Cancer Center. He received a BA in biology from Northeastern University, Boston (1963), a MA in biology from Wesleyan University, Middletown, Connecticut (1965) and a PhD in zoology from the University of Iowa, Iowa City (1969). He received Postdoctoral training in biochemistry at the National Institute for Medical Research, London (1969 to 1970) and SUNY Stony Brook (1970  to 1972) before becoming an assistant professor of biology at the University of South Florida (1972 to 1976) prior to moving to Washington in 1976 when he founded the Seattle Biomedical Research Institute (dba the Center for Infectious Disease Research). He is an expert on the molecular and cell biology of parasitic pathogens and is known for his groundbreaking studies of RNA editing, a novel fundamental genetic process. He led a consortium for the discovery of drugs for parasitic diseases and was a leader in an international consortium that sequenced and interpreted the genomes of three related parasites and currently leads a multi-institutional project studying human immune responses to malaria and HIV vaccines.

  • Award Name Award Description Awarded By Award Date
    Pioneer in Global Health Washington Global Health Alliance 2014
    Alice and C.C. Wang Award in Molecular Parasitology American Society for Biochemistry and Molecular Biology 2013
    Stoll-Stunkard Memorial Award and Lectureship American Society of Parasitologists 2002
    Denis Thienpont Prize For work in RNA Editing Royal Academies of Medicine of Belgium 1997
    NIAID Merit Award National Institutes of Health 1992 - 2002
    The Burroughs Wellcome Fund Molecular Parasitology Scholar Award 1988 - 1993
    The Burroughs Wellcome Fund Special Award in Molecular Parasitology 1986 - 1988
    Summer Faculty Research Fellowship American Society for Engineering Education/Navy 1985
  • Manuscripts in Refereed Journals

    • Rakshit S, Ahmed A, Adiga V, Sundararaj BK, Sahoo PN, Kenneth J, D'Souza G, Bonam W, Johnson C, Franken KL, Ottenhoff TH, Finak G, Gottardo R, Stuart KD, De Rosa SC, McElrath MJ, Vyakarnam A.
      BCG revaccination boosts adaptive polyfunctional Th1/Th17 and innate effectors in IGRA+ and IGRA- Indian adults.
      JCI Insight., 2019 Dec. 19 : 4(24)pii: 130540.
      https://www.ncbi.nlm.nih.gov/pubmed/31743110
    • Vijayan K, Cestari I, Mast FD, Glennon EKK, McDermott SM, Kain HS, Brokaw AM, Aitchison JD, Stuart K, Kaushansky A.
      Plasmodium Secretion Induces Hepatocyte Lysosome Exocytosis and Promotes Parasite Entry.
      iScience., 2019 Nov. 22 : 21603-611. PMCID:PMC6889558
      https://www.ncbi.nlm.nih.gov/pubmed/31731198
    • Healy SA, Murphy SC, Hume JCC, Shelton L, Kuntz S, Van Voorhis WC, Moodie Z, Metch B, Wang R, Silver-Brace T, Fishbaugher M, Kennedy M, Finney OC, Chaturvedi R, Hobbs CV, Warner-Lubin M, Talley AK, Wong-Madden S, Stuart K, Wald A, Kappe SH, Kublin JG, Duffy PE.
      Chemoprophylaxis vaccination: Phase 1 study to explore stage-specific immunity to Plasmodium falciparum in U.S. adults.
      Clin Infect Dis., 2019 Oct. 17 : Epub ahead
      https://www.ncbi.nlm.nih.gov/pubmed/31621832
    • McDermott SM, Carnes J, Stuart K.
      Editosome RNase III domain interactions are essential for editing and differ between life cycle stages in Trypanosoma brucei.
      RNA., 2019 Sept. : 25(9)1150-1163. PMCID:PMC6800513
      https://www.ncbi.nlm.nih.gov/pubmed/31171708
    • Cestari I, McLeland-Wieser H, Stuart K.
      Nuclear Phosphatidylinositol 5-Phosphatase Is Essential for Allelic Exclusion of Variant Surface Glycoprotein Genes in Trypanosomes.
      Mol Cell Biol., 2019 Jan. 16 : 39(3)pii: e00395-18. PMCID:PMC6336139
      https://www.ncbi.nlm.nih.gov/pubmed/30420356
    • Rothen J, Murie C, Carnes J, Anupama A, Abdulla S, Chemba M, Mpina M, Tanner M, Lee Sim BK, Hoffman SL, Gottardo R, Daubenberger C, Stuart K.
      Whole blood transcriptome changes following controlled human malaria infection in malaria pre-exposed volunteers correlate with parasite prepatent period.
      PLoS One., 2018 June 19 : 13(6)e0199392. PMCID:PMC6007927
      https://www.ncbi.nlm.nih.gov/pubmed/29920562
    • Cestari I, Anupama A, Stuart K.
      Inositol polyphosphate multikinase regulation of Trypanosoma brucei life stage development.
      Mol Biol Cell., 2018 May 1 : 29(9)1137-1152. PMCID:PMC5921579
      https://www.ncbi.nlm.nih.gov/pubmed/29514930
    • Carnes J, McDermott SM, Stuart K.
      RNase III Domain of KREPB9 and KREPB10 Association with Editosomes in Trypanosoma brucei.
      mSphere., 2018 Jan. 17 : 3(1)pii:e00585-17. PMCID:PMC5770545
      https://www.ncbi.nlm.nih.gov/pubmed/29359194
    • Moretti NS, Cestari I, Anupama A, Stuart K, Schenkman S.
      Comparative Proteomic Analysis of Lysine Acetylation in Trypanosomes.
      J Proteome Res., 2018 Jan. 5 : 17(1)374-385.
      https://www.ncbi.nlm.nih.gov/pubmed/29168382
    • McDermott SM, Stuart K.
      The essential functions of KREPB4 are developmentally distinct and required for endonuclease association with editosomes.
      RNA., 2017 Nov. : 23(11)1672-1684. PMCID:PMC5648035
      https://www.ncbi.nlm.nih.gov/pubmed/28802260
    • HIPC-CHI Signatures Project Team; HIPC-I Consortium.
      Multicohort analysis reveals baseline transcriptional predictors of influenza vaccination responses.
      Sci Immunol., 2017 Aug. 25 : 2(14)pii: eaal4656. PMCID:PMC5800877
      https://www.ncbi.nlm.nih.gov/pubmed/28842433
    • Mpina M, Maurice NJ, Yajima M, Slichter CK, Miller HW, Dutta M, McElrath MJ, Stuart KD, De Rosa SC, McNevin JP, Linsley PS, Abdulla S, Tanner M, Hoffman SL, Gottardo R, Daubenberger CA, Prlic M.
      Controlled Human Malaria Infection Leads to Long-Lasting Changes in Innate and Innate-like Lymphocyte Populations.
      J Immunol., 2017 July 1 : 199(1)107-118. PMCID:PMC5528886
      https://www.ncbi.nlm.nih.gov/pubmed/28576979
    • Carnes J, McDermott S, Anupama A, Oliver BG, Sather DN, Stuart K.
      In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases.
      Nucleic Acids Res. , 2017 May 5 : 45(8)4667-4686. PMCID:PMC5416837
      https://www.ncbi.nlm.nih.gov/pubmed/28334821
    • McDermott SM, Luo J, Carnes J, Ranish JA, Stuart K.
      The Architecture of Trypanosoma brucei editosomes.
      Proc Natl Acad Sci U S A., 2016 Oct. 18 : 113(42)E6476-E6485. PMCID:PMC5081628
      https://www.ncbi.nlm.nih.gov/pubmed/27708162
    • McDermott SM, Carnes J, Stuart K.
      Identification by Random Mutagenesis of Functional Domains in KREPB5 That Differentially Affect RNA Editing between Life Cycle Stages of Trypanosoma brucei.
      Mol Cell Biol., 2015 Dec. : 35(23)3945-61. PMCID:PMC4628071
      https://www.ncbi.nlm.nih.gov/pubmed/26370513
    • McDermott SM, Guo X, Carnes J, Stuart K.
      Differential Editosome Protein Function between Life Cycle Stages of Trypanosoma brucei.
      J Biol Chem., 2015 Oct. 9 : 290(41)24914-31. PMCID:PMC4599000
      https://www.ncbi.nlm.nih.gov/pubmed/26304125
    • Li Q, Leija C, Rijo-Ferreira F, Chen J, Cestari I, Stuart K, Tu BP, Phillips MA.
      GMP synthase is essential for viability and infectivity of Trypanosoma brucei despite a redundant purine salvage pathway.
      Mol Microbiol., 2015 Sept. : 51006-20. PMCID:PMC4550530
      https://www.ncbi.nlm.nih.gov/pubmed/26043892
    • Cestari I, Stuart K.
      Inositol phosphate pathway controls transcription of telomeric expression sites in trypanosomes.
      Proc Natl Acad Sci U S A., 2015 May 26 : 112(21)E2803-12. PMCID:PMC4450425
      https://www.ncbi.nlm.nih.gov/pubmed/25964327
    • Phan IQ, Davies DR, Moretti NS, Shanmugam D, Cestari I, Anupama A, Fairman JW, Edwards TE, Stuart K, Schenkman S, Myler PJ.
      Iron superoxide dismutases in eukaryotic pathogens: new insights from Apicomplexa and Trypanosoma structures.
      Acta Crystallogr F Struct Biol Commun., 2015 May : 71(Pt 5)615-21. PMCID:PMC4427173
      https://www.ncbi.nlm.nih.gov/pubmed/25961325
    • Stuart K.
      Personal reflections on RNA: an emphasis on trypanosomes.
      RNA., 2015 April : 21(4)745-6. PMCID:PMC4371360
      https://www.ncbi.nlm.nih.gov/pubmed/25780218
    • Carnes J, Anupama A, Balmer O, Jackson A, Lewis M, Brown R, Cestari I, Desquesnes M, Gendrin C, Hertz-Fowler C, Imamura H, Ivens A, Kořený L, Lai DH, MacLeod A, McDermott SM, Merritt C, Monnerat S, Moon W, Myler P, Phan I, Ramasamy G, Sivam D, Lun ZR, Lukeš J, Stuart K, Schnaufer A.
      Genome and phylogenetic analyses of Trypanosoma evansi reveal extensive similarity to T. bruceiand multiple independent origins for dyskinetoplasty.
      PLoS Negl Trop Dis., 2015 Jan. 8 : 9(1)e3404. PMCID:PMC4288722
      https://www.ncbi.nlm.nih.gov/pubmed/25568942
    • Carnes J, Lerch M, Kurtz I, Stuart K.
      Bloodstream form Trypanosoma brucei do not require mRPN1 for gRNA processing.
      RNA., 2015 Jan. : 21(1)28-35. PMCID:PMC4274635
      https://www.ncbi.nlm.nih.gov/pubmed/25404564
    • Demir O, Labaied M, Merritt C, Stuart K, Amaro RE.
      Computer-aided discovery of Trypanosoma brucei RNA-editing terminal uridylyl transferase 2 inhibitors.
      Chem Biol Drug Des., 2014 Aug. : 84(2)131-9. PMCID:PMC4317284
      https://www.ncbi.nlm.nih.gov/pubmed/24903413
    • Sekar A, Merritt C, Baugh L, Stuart K, Myler PJ.
      Tb927.10.6900 encodes the glucosyltransferase involved in synthesis of base J in Trypanosoma brucei.
      Mol Biochem Parasitol., 2014 Aug. : 196(1)9-11. PMCID:PMC4206709
      https://www.ncbi.nlm.nih.gov/pubmed/25064607

    Other Publications

    • Cestari I, Stuart K.
      Transcriptional Regulation of Telomeric Expression Sites and Antigenic Variation in Trypanosomes.
      Curr Genomics., 2018 Feb. : 19(2)119-132. Review. PMCID:PMC5814960
      https://www.ncbi.nlm.nih.gov/pubmed/29491740
    • Merritt C, Silva LE, Tanner AL, Stuart K, Pollastri MP.
      Kinases as druggable targets in trypanosomatid protozoan parasites.
      Chem Rev., 2014 Nov. 26 : 114(22)11280-304. Review. PMCID:PMC4254031
      https://www.ncbi.nlm.nih.gov/pubmed/26443079

  • Grant Title Grantor Amount Award Date
    Immune Responses to Malaria and HIV Infection and Immunization - U19 AI128914-01 (Stuart) NIH/NIAID $17,038,149 Total Award July 2017 - June 2022
    Mitochondrial DNA of Normal and Mutant Trypanosomes - R01 AI014102 (Stuart) NIH/NIAID $4,535,937 Total Sept. 1978 - April 2023

Overview

Research Description

Research in the Stuart Lab is focused on protozoan pathogens and the diseases that they cause. These include malaria which is caused by Plasmodium parasites and Human African Trypanosomiasis (sleeping sickness), Chagas disease and Leishmaniasis that are caused by three Trypanosomatid parasites. The lab investigates molecular and cellular processes of the parasites and immune responses to infection and vaccines in order to develop drugs, vaccines and diagnostics that are needed. Read more about the Stuart Lab.

Research Focus Area

Biotechnology, Chagas Disease, Genetic Engineering, Genetics, Host-Pathogen Interaction, Immunology, Malaria, Global Health, Infectious Disease, Leishmaniasis, Systems Biology, Trypanosomiasis