CGIDR Imaging Core
The Imaging Core located in the Center for Global Infectious Disease Research provides the tools for capturing and analyzing images which allows researchers to describe cell changes and function under varying conditions and to characterize individual molecules at both the cellular and biochemical level.
Imaging Core Equipment
- GE Healthcare DeltaVision Elite microscope: This fully-automated, high resolution microscope system is capable of 3D live or fixed cell imaging in real time. It is equipped with DIC optics for acquiring visible light images and up to seven channels for imaging fluorescently labeled cells. This system includes multi-wavelength automated image capture for DAPI, FITC, TRITC, mCherry, CFP, YFP, and Cy-5 dyes. The SoftWorx software package comes with deconvolution capabilities and other image analysis features to create the highest quality images for publication.
- Leica SP2 laser scanning confocal microscope: This microscope has four lasers, a motorized Z stage, manual X-Y stage, and integrated software control. The resolution is sufficient for sub-cellular organelle analysis. The integrated software supports multicolor fluorescent studies, FRET, FRAP, 3D image reconstruction and analysis, time-lapse imaging, spectral analysis and color separation. Also included is phase contrast and DIC optics for brightfield work.
- Nikon Eclipse E600 upright microscope: This scope has phase contrast optics and three filters for fluorescence imaging. It is equipped with both a high speed Cool Snap CCD camera used for fluorescence and phase contrast images, as well as a Qimaging true color camera used for chromatic staining in tissue samples. Metamorph analysis software is included.
- IVIS Lumina II imaging system: This instrument from Caliper Life Sciences provides an expandable, sensitive imaging system for both fluorescent and bioluminescent imaging in vivo. The system includes a highly sensitive CCD camera, light-tight imaging chamber and complete automation and analysis capabilities.
- Nikon Ti-E inverted microscope: This scope is a fully automated inverted microscope system equipped for phase contrast, DIC and fluorescence imaging. It houses 6 objective lenses, a high quality digital camera that can take both true color and monochrome images, and 4 filters for fluorescence (FITC, DAPI, Texas Red and Cy5). The system is equipped with an environmental chamber for control of temperature, CO2 and humidity in live cell imaging. The motorized stage, motorized filter wheel and motorized objective wheel allows for automated scanning of plates, slides, and petri dishes. It operates with Nikon Elements 6D control/analysis software.
- Other downstream image analysis software available includes Imaris 3D imaging software for data visualization, segmentation, analysis and interpretation of 3D and 4D microscopy datasets, and SVI Huygens software for deconvolution of 3D data sets from any of the above platforms.
Request Services and Equipment
Imaging Core equipment and training are available for Seattle Children’s researchers. For more information on pricing and scheduling, or to discuss your upcoming project, contact the core manager, Tracy Mitchell.