Skip to nav Skip to content

COVID-19 AdvisoryVisitors limited to only one parent/caregiver at all clinics and no siblings allowed: learn more. Telehealth update.

Cilial Genes and Polycystic Kidney Disease

Kidneys

Several of our positional cloning efforts have uncovered genes that play in cilial physiology. For example, Nek8, which is required for normal left-right patterning and which is associated with a cystic kidney disorder, is the only kinase reported to localize to the cilial axoneme. Ttc21b is a component of a complex of proteins that mediate retrograde intraflagellar transport, and our analysis of a conditional mutant have implicated hedgehog signaling in kidney cystogenesis. Other cilial genes we have identified have been implicated in craniofacial development and hydrocephalus.

While the mutants we have studied are useful models, the most common causes of cystic kidney disease in humans are due to mutations in the polycystins PC1 and PC2. Despite many years of study, the pathological mechanism of these disorders is not understood. Null mutations in these genes are embryonic lethal in mice; however, an engineered hypomorphic mutant shows progressive cystic disease in adult mice that provides an important experimental model (Hopp et al., J. Clinical Investigation, 2012). We are studying this line and our other cystic kidney mutants using methods including single-cell RNA sequencing analysis in order to assess the earliest events that occur in this disorder.

By clicking “Accept All Cookies,” you agree to the storing of cookies on your device to enhance site navigation, analyze site usage and assist in marketing efforts. For more information, see Website Privacy.

Accept All Cookies