Ashley Vaughan, PhD

Infectious Disease Research

Children's Title: Research Assistant Professor

Research Center: Center for Global Infectious Disease Research

  • Ashley Vaughan, PhD, is a research assistant professor. He received his PhD from the London School of Hygiene and Tropical Medicine. During his tenure in the Kappe Lab, he showed the importance of the parasite’s fatty acid synthetic pathway for sporozoite and liver stage maturation. He also researches how to elicit the most protective immune response after vaccination with genetically attenuated parasites. Ashley’s collaborations with Sebastian Mikolajczak led to significant advances in the use of human-liver chimeric mouse models in studying malaria. This includes complete liver stage development and the transition to blood stage malaria in the mouse for the human malarias Plasmodium falciparum and Plasmodium vivax. He has also used this mouse model for the creation of experimental Plasmodium falciparum genetic crosses, a significant advance that should aid in our understanding of Plasmodium falciparum drug resistance. Ashley continues to be fascinated by basic parasite pre-erythrocytic biology and uses rodent malaria and parasite transgenesis to understand how the parasite interacts with its vector and host during sporozoite and liver stage development. Outside of the lab, he is a keen hiker, traveler, gardener and scuba diver, pastimes he enjoys with and without his husband, Rafael.

  • Award Name Award Description Awarded By Award Date
    New Investigator Award to attend the Third Annual Conference on Arteriosclerosis Thrombosis and Vascular Biology 2002
    New Investigator Award to attend the Second Annual Conference on Arteriosclerosis Thrombosis and Vascular Biology 2001
  • Manuscripts in Refereed Journals

    • Flannery EL, Markus MB, Vaughan AM.
      Plasmodium vivax.
      Trends Parasitol., 2019 July : 35(7)583-584
      https://www.ncbi.nlm.nih.gov/pubmed/31176582
    • Seilie AM, Chang M, Hanron AE, Billman ZP, Stone BC, Zhou K, Olsen TM, Daza G, Ortega J, Cruz KR, Smith N, Healy SA, Neal J, Wallis CK, Shelton L, Mankowski TV, Wong-Madden S, Mikolajczak SA, Vaughan AM, Kappe SHI, Fishbaugher M, Betz W, Kennedy M, Hume JCC, Talley AK, Hoffman SL, Chakravarty S, Sim BKL, Richie TL, Wald A, Plowe CV, Lyke KE, Adams M, Fahle GA, Cowan EP, Duffy PE, Kublin JG, Murphy SC.
      Beyond Blood Smears: Qualification of Plasmodium 18S rRNA as a Biomarker for Controlled Human Malaria Infections.
      Am J Trop Med Hyg., 2019 June : 100(6)1466-1476 PMCID:PMC6553913
      https://www.ncbi.nlm.nih.gov/pubmed/31017084
    • Gupta DK, Dembele L, Voorberg-van der Wel A, Roma G, Yip A, Chuenchob V, Kangwanrangsan N, Ishino T, Vaughan AM, Kappe SH, Flannery EL, Sattabongkot J, Mikolajczak S, Bifani P, Kocken CH, Diagana TT.
      The Plasmodium liver-specific protein 2 (LISP2) is an early marker of liver stage development.
      Elife., 2019 May 16 : 8e43362 PMCID:PMC6542585
      https://www.ncbi.nlm.nih.gov/pubmed/31094679
    • Arredondo SA, Swearingen KE, Martinson T, Steel R, Dankwa DA, Harupa A, Camargo N, Betz W, Vigdorovich V, Oliver BG, Kangwanrangsan N, Ishino T, Sather N, Mikolajczak S, Vaughan AM, Torii M, Moritz RL, Kappe SHI.
      The Micronemal Plasmodium Proteins P36 and P52 Act in Concert to Establish the Replication-Permissive Compartment Within Infected Hepatocytes.
      Front Cell Infect Microbiol., 2018 Nov. 27 : 8413 PMCID:PMC6280682
      https://www.ncbi.nlm.nih.gov/pubmed/30547015
    • Zander RA, Vijay R, Pack AD, Guthmiller JJ, Graham AC, Lindner SE, Vaughan AM, Kappe SHI, Butler NS.
      Th1-like Plasmodium-Specific Memory CD4+ T Cells Support Humoral Immunity.
      Cell Rep., 2018 April 24 : 23(4)1230-1237
      https://www.ncbi.nlm.nih.gov/pubmed/29694898
    • Vaughan AM, Sack BK, Dankwa D, Minkah N, Nguyen T, Cardamone H, Kappe SHI.
      A Plasmodium Parasite with Complete Late Liver Stage Arrest Protects against Preerythrocytic and Erythrocytic Stage Infection in Mice.
      Infect Immun., 2018 April 23 : 86(5)pii:e00088-18 PMCID:PMC5913857
      https://www.ncbi.nlm.nih.gov/pubmed/29440367
    • Itsara LS, Zhou Y, Do J, Dungel S, Fishbaugher ME, Betz WW, Nguyen T, Navarro MJ, Flannery EL, Vaughan AM, Kappe SHI, Ghosh AK.
      PfCap380 as a marker for Plasmodium falciparum oocyst development in vivo and in vitro.
      Malar J., 2018 April 2 : 17(1)135 PMCID:PMC5880026
      https://www.ncbi.nlm.nih.gov/pubmed/29609625
    • Steel RWJ, Pei Y, Camargo N, Kaushansky A, Dankwa DA, Martinson T, Nguyen T, Betz W, Cardamone H, Vigdorovich V, Dambrauskas N, Carbonetti S, Vaughan AM, Sather DN, Kappe SHI.
      Plasmodium yoelii S4/CelTOS is important for sporozoite gliding motility and cell traversal.
      Cell Microbiol., 2018 : 20(4)
      https://www.ncbi.nlm.nih.gov/pubmed/29253313
    • Zander RA, Vijay R, Pack AD, Guthmiller JJ, Graham AC, Lindner SE, Vaughan AM, Kappe SHI, Butler NS.
      Th1-like Plasmodium-Specific Memory CD4+ T Cells Support Humoral Immunity.
      Cell Rep., 2017 Nov. 14 : 21(7)1839-1852. Erratum in: Cell Rep. 2018 Apr 24;23(4) PMCID:PMC5693336
      https://www.ncbi.nlm.nih.gov/pubmed/29141217
    • Sack BK, Mikolajczak SA, Fishbaugher M, Vaughan AM, Flannery EL, Nguyen T, Betz W, Jane Navarro M, Foquet L, Steel RWJ, Billman ZP, Murphy SC, Hoffman SL, Chakravarty S, Sim BKL, Behet M, Reuling IJ, Walk J, Scholzen A, Sauerwein RW, Ishizuka AS, Flynn B, Seder RA, Kappe SHI.
      Humoral protection against mosquito bite-transmitted Plasmodium falciparum infection in humanized mice.
      NPJ Vaccines., 2017 Oct. 9 : 227 PMCID:PMC5634440
      https://www.ncbi.nlm.nih.gov/pubmed/29263882
    • Swearingen KE, Lindner SE, Flannery EL, Vaughan AM, Morrison RD, Patrapuvich R, Koepfli C, Muller I, Jex A, Moritz RL, Kappe SHI, Sattabongkot J, Mikolajczak SA.
      Proteogenomic analysis of the total and surface-exposed proteomes of Plasmodium vivax salivary gland sporozoites.
      PLoS Negl Trop Dis., 2017 July 31 : 11(7)e0005791 PMCID:PMC5552340
      https://www.ncbi.nlm.nih.gov/pubmed/28759593
    • Kublin JG, Mikolajczak SA, Sack BK, Fishbaugher ME, Seilie A, Shelton L, VonGoedert T, Firat M, Magee S, Fritzen E, Betz W, Kain HS, Dankwa DA, Steel RW, Vaughan AM, Noah Sather D, Murphy SC, Kappe SH.
      Complete attenuation of genetically engineered Plasmodium falciparum sporozoites in human subjects.
      Sci Transl Med., 2017 Jan. 4 : 4(9)371
      https://www.ncbi.nlm.nih.gov/pubmed/28053159
    • Keitany GJ, Kim KS, Krishnamurty AT, Hondowicz BD, Hahn WO, Dambrauskas N, Sather DN, Vaughan AM, Kappe SHI, Pepper M.
      Blood Stage Malaria Disrupts Humoral Immunity to the Pre-erythrocytic Stage Circumsporozoite Protein.
      Cell Rep., 2016 Dec. 20 : 17(12)3193-3205 PMCID:PMC5476299
      https://www.ncbi.nlm.nih.gov/pubmed/28009289
    • Swearingen KE, Lindner SE, Shi L, Shears MJ, Harupa A, Hopp CS, Vaughan AM, Springer TA, Moritz RL, Kappe SH, Sinnis P.
      Interrogating the Plasmodium Sporozoite Surface: Identification of Surface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP by Mass Spectrometry-Based Proteomics.
      PLoS Pathog., 2016 April 29 : 12(4)e1005606 PMCID:PMC4851412
      https://www.ncbi.nlm.nih.gov/pubmed/27128092
    • Dankwa DA, Davis MJ, Kappe SHI, Vaughan AM.
      A Plasmodium yoelii Mei2-Like RNA Binding Protein Is Essential for Completion of Liver Stage Schizogony.
      Infect Immun., 2016 April 22 : 84(5)1336-1345 PMCID:PMC4862717
      https://www.ncbi.nlm.nih.gov/pubmed/26883588
    • Vaughan AM, Pinapati RS, Cheeseman IH, Camargo N, Fishbaugher M, Checkley LA, Nair S, Hutyra CA, Nosten FH, Anderson TJ, Ferdig MT, Kappe SH.
      Plasmodium falciparum genetic crosses in a humanized mouse model.
      Nat Methods., 2015 July : 12(7)631-3 PMCID:PMC4547688
      https://www.ncbi.nlm.nih.gov/pubmed/26030447
    • Sack BK, Keitany GJ, Vaughan AM, Miller JL, Wang R, Kappe SH.
      Mechanisms of stage-transcending protection following immunization of mice with late liver stage-arresting genetically attenuated malaria parasites.
      PLoS Pathog., 2015 May 14 : 11(5)e1004855 PMCID:PMC4431720
      https://www.ncbi.nlm.nih.gov/pubmed/25974076
    • Mikolajczak SA, Vaughan AM, Kangwanrangsan N, Roobsoong W, Fishbaugher M, Yimamnuaychok N, Rezakhani N, Lakshmanan V, Singh N, Kaushansky A, Camargo N, Baldwin M, Lindner SE, Adams JH, Sattabongkot J, Kappe SH.
      Plasmodium vivax liver stage development and hypnozoite persistence in human liver-chimeric mice.
      Cell Host Microbe., 2015 April 8 : 17(4)526-35 PMCID:PMC5299596
      https://www.ncbi.nlm.nih.gov/pubmed/25800544
    • Lakshmanan V, Fishbaugher ME, Morrison B, Baldwin M, Macarulay M, Vaughan AM, Mikolajczak SA, Kappe SH.
      Cyclic GMP balance is critical for malaria parasite transmission from the mosquito to the mammalian host.
      MBio., 2015 March 17 : 6(2)e02330 PMCID:PMC4453516
      https://www.ncbi.nlm.nih.gov/pubmed/25784701
    • Murray SA, Mohar I, Miller JL, Brempelis KJ, Vaughan AM, Kappe SH, Crispe IN.
      CD40 is required for protective immunity against liver stage Plasmodium infection.
      J Immunol., 2015 March 1 : 194(5)2268-79 PMCID:PMC4340756
      https://www.ncbi.nlm.nih.gov/pubmed/25646303
    • Kaushansky A, Austin LS, Mikolajczak SA, Lo FY, Miller JL, Douglass AN, Arang N, Vaughan AM, Gardner MJ, Kappe SH.
      Susceptibility to Plasmodium yoelii preerythrocytic infection in BALB/c substrains is determined at the point of hepatocyte invasion.
      Infect Immun., 2015 Jan. : 83(1)39-47 PMCID:PMC4288894
      https://www.ncbi.nlm.nih.gov/pubmed/25312960
    • Keitany GJ, Sack B, Smithers H, Chen L, Jang IK, Sebastian L, Gupta M, Sather DN, Vignali M, Vaughan AM, Kappe SH, Wang R.
      Immunization of mice with live-attenuated late liver stage-arresting Plasmodium yoelii parasites generates protective antibody responses to preerythrocytic stages of malaria.
      Infect Immun., 2014 Dec. : 82(12)5143-53 PMCID:PMC4249261
      https://www.ncbi.nlm.nih.gov/pubmed/25267837
    • Stone WJ, Churcher TS, Graumans W, van Gemert GJ, Vos MW, Lanke KH, van de Vegte-Bolmer MG, Siebelink-Stoter R, Dechering KJ, Vaughan AM, Camargo N, Kappe SH, Sauerwein RW, Bousema T.
      A scalable assessment of Plasmodium falciparum transmission in the standard membrane-feeding assay, using transgenic parasites expressing green fluorescent protein-luciferase.
      J Infect Dis., 2014 Nov. 1 : 210(9)1456-63
      https://www.ncbi.nlm.nih.gov/pubmed/24829466
    • van Schaijk BC, Kumar TR, Vos MW, Richman A, van Gemert GJ, Li T, Eappen AG, Williamson KC, Morahan BJ, Fishbaugher M, Kennedy M, Camargo N, Khan SM, Janse CJ, Sim KL, Hoffman SL, Kappe SH, Sauerwein RW, Fidock DA, Vaughan AM.
      Type II fatty acid biosynthesis is essential for Plasmodium falciparum sporozoite development in the midgut of Anopheles mosquitoes.
      Eukaryot Cell., 2014 May : 13(5)550-9 PMCID:PMC4060470
      https://www.ncbi.nlm.nih.gov/pubmed/24297444
    • Miller JL, Sack BK, Baldwin M, Vaughan AM, Kappe SHI.
      Interferon-mediated innate immune responses against malaria parasite liver stages.
      Cell Rep., 2014 April 24 : 7(2)436-447
      https://www.ncbi.nlm.nih.gov/pubmed/24703850
    • Lindner SE, Sartain MJ, Hayes K, Harupa A, Moritz RL, Kappe SH, Vaughan AM.
      Enzymes involved in plastid-targeted phosphatidic acid synthesis are essential for Plasmodium yoelii liver-stage development.
      Mol Microbiol., 2014 Feb. : 91(4)679-93 PMCID:PMC3925071
      https://www.ncbi.nlm.nih.gov/pubmed/24330260
    • Sack BK, Miller JL, Vaughan AM, Douglass A, Kaushansky A, Mikolajczak S, Coppi A, Gonzalez-Aseguinolaza G, Tsuji M, Zavala F, Sinnis P, Kappe SH.
      Model for in vivo assessment of humoral protection against malaria sporozoite challenge by passive transfer of monoclonal antibodies and immune serum.
      Infect Immun., 2014 Feb. : 82(2)808-17 PMCID:PMC3911395
      https://www.ncbi.nlm.nih.gov/pubmed/24478094
    • Cobbold SA, Vaughan AM, Lewis IA, Painter HJ, Camargo N, Perlman DH, Fishbaugher M, Healer J, Cowman AF, Kappe SH, Llinás M.
      Kinetic flux profiling elucidates two independent acetyl-CoA biosynthetic pathways in Plasmodium falciparum.
      J Biol Chem., 2013 Dec. 20 : 288(51)36338-50 PMCID:PMC3868748
      https://www.ncbi.nlm.nih.gov/pubmed/24163372
    • Pei Y, Miller JL, Lindner SE, Vaughan AM, Torii M, Kappe SHI.
      Plasmodium yoelii inhibitor of cysteine proteases is exported to exomembrane structures and interacts with yoelipain-2 during asexual blood-stage development.
      Cell Microbiol., 2013 Sept. : 15(9)1508-1526 PMCID:PMC3907115
      https://www.ncbi.nlm.nih.gov/pubmed/23421981
    • Lindner SE, Mikolajczak SA, Vaughan AM, Moon W, Joyce BR, Sullivan WJ Jr, Kappe SH.
      Perturbations of Plasmodium Puf2 expression and RNA-seq of Puf2-deficient sporozoites reveal a critical role in maintaining RNA homeostasis and parasite transmissibility.
      Cell Microbiol., 2013 July : 15(7)1266-83 PMCID:PMC3815636
      https://www.ncbi.nlm.nih.gov/pubmed/23356439
    • Lindner SE, Swearingen KE, Harupa A, Vaughan AM, Sinnis P, Moritz RL, Kappe SH.
      Total and putative surface proteomics of malaria parasite salivary gland sporozoites.
      Mol Cell Proteomics., 2013 May : 12(5)1127-43 PMCID:PMC3650326
      https://www.ncbi.nlm.nih.gov/pubmed/23325771
    • Miller JL, Murray S, Vaughan AM, Harupa A, Sack B, Baldwin M, Crispe IN, Kappe SH.
      Quantitative bioluminescent imaging of pre-erythrocytic malaria parasite infection using luciferase-expressing Plasmodium yoelii.
      PLoS One., 2013 April 11 : 8(4)e60820 PMCID:PMC3623966
      https://www.ncbi.nlm.nih.gov/pubmed/23593316
    • Kaushansky A, Ye AS, Austin LS, Mikolajczak SA, Vaughan AM, Camargo N, Metzger PG, Douglass AN, MacBeath G, Kappe SH.
      Suppression of host p53 is critical for Plasmodium liver-stage infection.
      Cell Rep., 2013 March 28 : 3(3)630-7 PMCID:PMC3619000
      https://www.ncbi.nlm.nih.gov/pubmed/23478020
    • Vaughan AM, Mikolajczak SA, Camargo N, Lakshmanan V, Kennedy M, Lindner SE, Miller JL, Hume JC, Kappe SH.
      A transgenic Plasmodium falciparum NF54 strain that expresses GFP-luciferase throughout the parasite life cycle.
      Mol Biochem Parasitol. , 2012 Dec. : 186(2)143-7
      https://www.ncbi.nlm.nih.gov/pubmed/23107927
    • Kennedy M, Fishbaugher ME, Vaughan AM, Patrapuvich R, Boonhok R, Yimamnuaychok N, Rezakhani N, Metzger P, Ponpuak M, Sattabongkot J, Kappe SH, Hume JC, Lindner SE.
      A rapid and scalable density gradient purification method for Plasmodium sporozoites.
      Malar J., 2012 Dec. : 11421 PMCID:PMC3543293
      https://www.ncbi.nlm.nih.gov/pubmed/23244590
    • Vaughan AM, Mikolajczak SA, Wilson EM, Grompe M, Kaushansky A, Camargo N, Bial J, Ploss A, Kappe SH.
      Complete Plasmodium falciparum liver-stage development in liver-chimeric mice.
      J Clin Invest., 2012 Oct. : 122(10)3618-28 PMCID:PMC3461911
      https://www.ncbi.nlm.nih.gov/pubmed/22996664
    • Vaughan AM, Kappe SH, Ploss A, Mikolajczak SA.
      Development of humanized mouse models to study human malaria parasite infection.
      Future Microbiol., 2012 May : 7(5)657-65 PMCID:PMC3848604
      https://www.ncbi.nlm.nih.gov/pubmed/22568719
    • MacKellar DC, Vaughan AM, Aly AS, DeLeon S, Kappe SH.
      A systematic analysis of the early transcribed membrane protein family throughout the life cycle of Plasmodium yoelii.
      Cell Microbiol., 2011 Nov. : 13(11)1755-67 PMCID:PMC3328200
      https://www.ncbi.nlm.nih.gov/pubmed/21819513
    • Butler NS, Schmidt NW, Vaughan AM, Aly AS, Kappe SH, Harty JT.
      Superior antimalarial immunity after vaccination with late liver stage-arresting genetically attenuated parasites.
      Cell Host Microbe., 2011 June 16 : 9(6)451-62 PMCID:PMC3117254
      https://www.ncbi.nlm.nih.gov/pubmed/21669394
    • de Beer MC, Ji A, Jahangiri A, Vaughan AM, de Beer FC, van der Westhuyzen DR, Webb NR.
      ATP binding cassette G1-dependent cholesterol efflux during inflammation.
      J Lipid Res., 2011 Feb. : 52(2)345-53. PMCID:PMC3023555
      https://www.ncbi.nlm.nih.gov/pubmed/21138980
    • Tchoua U, Rosales C, Tang D, Gillard BK, Vaughan A, Lin HY, Courtney HS, Pownall HJ.
      Serum opacity factor enhances HDL-mediated cholesterol efflux, esterification and anti inflammatory effects.
      Lipids., 2010 Dec. : 45(12)1117-26 PMCID:PMC3036000
      https://www.ncbi.nlm.nih.gov/pubmed/20972840
    • Jacobs-Lorena VY, Mikolajczak SA, Labaied M, Vaughan AM, Kappe SH.
      A dispensable Plasmodium locus for stable transgene expression.
      Mol Biochem Parasitol., 2010 May : 171(1)40-4 PMCID:PMC2839042
      https://www.ncbi.nlm.nih.gov/pubmed/20045029
    • Mikolajczak SA, Vaughan AM, Soliman JM, Kappe SH.
      A genetically attenuated parasite vaccine does not require liver stage persistence to elicit sterile protective immunity against sporozoite-induced malaria in mice.
      J Infect Dis., 2010 April 15 : 201(8)1270-1. Author reply: 1271-2.
      https://www.ncbi.nlm.nih.gov/pubmed/20225958
    • Pei Y, Tarun AS, Vaughan AM, Herman RW, Soliman JM, Erickson-Wayman A, Kappe SH.
      Plasmodium pyruvate dehydrogenase activity is only essential for the parasite's progression from liver infection to blood infection.
      Mol Microbiol., 2010 Feb. : 75(4)957-71
      https://www.ncbi.nlm.nih.gov/pubmed/20487290
    • Tang C, Liu Y, Kessler PS, Vaughan AM, Oram JF.
      The macrophage cholesterol exporter ABCA1 functions as an anti-inflammatory receptor.
      J Biol Chem., 2009 Nov. 20 : 284(47)32336-43. PMCID:PMC2781648
      https://www.ncbi.nlm.nih.gov/pubmed/19783654
    • Vaughan AM, O'Neill MT, Tarun AS, Camargo N, Phuong TM, Aly AS, Cowman AF, Kappe SH.
      Type II fatty acid synthesis is essential only for malaria parasite late liver stage development.
      Cell Microbiol., 2009 March : 11(3)506-20. PMCID:PMC2688669
      https://www.ncbi.nlm.nih.gov/pubmed/19068099
    • Vaughan AM, Tang C, Oram JF.
      ABCA1 mutants reveal an interdependency between lipid export function, apoA-I binding activity, and Janus kinase 2 activation.
      J Lipid Res., 2009 Feb. : 50(2)285-92. PMCID:PMC2636916
      https://www.ncbi.nlm.nih.gov/pubmed/18776170
    • Sankaranarayanan S, Oram JF, Asztalos BF, Vaughan AM, Lund-Katz S, Adorni MP, Phillips MC, Rothblat GH.
      Effects of acceptor composition and mechanism of ABCG1-mediated cellular free cholesterol efflux.
      J Lipid Res., 2009 Feb. : 50(2)275-84. PMCID:PMC2636919
      https://www.ncbi.nlm.nih.gov/pubmed/18827283
    • Vaughan A, Chiu SY, Ramasamy G, Li L, Gardner MJ, Tarun AS, Kappe SH, Peng X.
      Assessment and improvement of the Plasmodium yoelii yoelii genome annotation through comparative analysis.
      Bioinformatics, 2008 July 1 : 24(13)i383-9. PMCID:PMC2718618
      https://www.ncbi.nlm.nih.gov/pubmed/18586738
    • Mikolajczak SA, Aly AS, Dumpit RF, Vaughan AM, Kappe SH.
      An efficient strategy for gene targeting and phenotypic assessment in the Plasmodium yoelii rodent malaria model.
      Mol Biochem Parasitol., 2008 April : 158(2)213-6.
      https://www.ncbi.nlm.nih.gov/pubmed/18242728
    • Landry YD, Denis M, Nandi S, Bell S, Vaughan AM, Zha X.
      ATP-binding cassette transporter A1 expression disrupts raft membrane microdomains through its ATPase-related functions.
      J Biol Chem., 2006 Nov. 24 : 281(47)36091-101.
      https://www.ncbi.nlm.nih.gov/pubmed/16984907
    • Vaughan AM, Oram JF.
      ABCA1 and ABCG1 or ABCG4 act sequentially to remove cellular cholesterol and generate cholesterol-rich HDL.
      J Lipid Res., 2006 Nov. : 47(11)2433-43.
      https://www.ncbi.nlm.nih.gov/pubmed/16902247
    • Tang C, Vaughan AM, Anantharamaiah GM, Oram JF.
      Janus kinase 2 modulates the lipid-removing but not protein-stabilizing interactions of amphipathic helices with ABCA1.
      J Lipid Res., 2006 Jan. : 47(1)107-14.
      https://www.ncbi.nlm.nih.gov/pubmed/16210729
    • Amenya DA, Koekemoer LL, Vaughan A, Morgan JC, Brooke BD, Hunt RH, Ranson H, Hemingway J, Coetzee M.
      Isolation and sequence analysis of P450 genes from a pyrethroid resistant colony of the major malaria vector Anopheles funestus.
      DNA Seq., 2005 Dec. : 16(6)437-45.
      https://www.ncbi.nlm.nih.gov/pubmed/16287623
    • Vaughan AM, Oram JF.
      ABCG1 redistributes cell cholesterol to domains removable by high density lipoprotein but not by lipid-depleted apolipoproteins.
      J Biol Chem., 2005 Aug. 26 : 280(34)30150-7.
      https://www.ncbi.nlm.nih.gov/pubmed/15994327
    • David JP, Strode C, Vontas J, Nikou D, Vaughan A, Pignatelli PM, Louis C, Hemingway J, Ranson H.
      The Anopheles gambiae detoxification chip: a highly specific microarray to study metabolic-based insecticide resistance in malaria vectors.
      Proc Natl Acad Sci USA., 2005 March 15 : 102(11)4080-4 PMCID:PMC554807
      https://www.ncbi.nlm.nih.gov/pubmed/15753317
    • Ranson H, Paton MG, Jensen B, McCarroll L, Vaughan A, Hogan JR, Hemingway J, Collins FH.
      Genetic mapping of genes conferring permethrin resistance in the malaria vector, Anopheles gambiae.
      Insect Mol Biol., 2004 Aug. : 13(4)379-86
      https://www.ncbi.nlm.nih.gov/pubmed/15271210
    • Tang C, Vaughan AM, Oram JF.
      Janus kinase 2 modulates the apolipoprotein interactions with ABCA1 required for removing cellular cholesterol.
      J Biol Chem., 2004 Feb. 27 : 279(9)7622-8.
      https://www.ncbi.nlm.nih.gov/pubmed/14668333
    • Oram JF, Wolfbauer G, Vaughan AM, Tang C, Albers JJ.
      Phospholipid transfer protein interacts with and stabilizes ATP-binding cassette transporter A1 and enhances cholesterol efflux from cells.
      J Biol Chem., 2003 : 278(52)52379-85
      https://www.ncbi.nlm.nih.gov/pubmed/14559902
    • Vaughan AM, Oram JF.
      ABCA1 redistributes membrane cholesterol independent of apolipoprotein interactions.
      J Lipid Res., 2003 July : 44(7)1373-80.
      https://www.ncbi.nlm.nih.gov/pubmed/12700343
    • Oram JF, Vaughan AM, Stocker R.
      ATP-binding cassette transporter A1 mediates cellular secretion of alpha-tocopherol.
      J Biol Chem. , 2001 Oct. 26 : 276(43)39898-902.
      https://www.ncbi.nlm.nih.gov/pubmed/11546785
    • Hemingway J, Coleman M, Paton M, McCarroll L, Vaughan A, Desilva D.
      Aldehyde oxidase is coamplified with the World's most common Culex mosquito insecticide resistance-associated esterases.
      Insect Mol Biol., 2000 Feb. : 9(1)93-9.
      https://www.ncbi.nlm.nih.gov/pubmed/10672076
    • Lawn RM, Wade DP, Garvin MR, Wang X, Schwartz K, Porter JG, Seilhamer JJ, Vaughan AM, Oram JF.
      The Tangier disease gene product ABC1 controls the cellular apolipoprotein-mediated lipid removal pathway.
      J Clin Invest., 1999 Oct. : 104(8)R25-31. PMCID:PMC481052
      https://www.ncbi.nlm.nih.gov/pubmed/10525055
    • Karunaratne SH, Vaughan A, Paton MG, Hemingway J.
      Amplification of a serine esterase gene is involved in insecticide resistance in Sri Lankan Culex tritaeniorhynchus.
      Insect Mol Biol., 1998 Nov. : 7(4)307-15.
      https://www.ncbi.nlm.nih.gov/pubmed/9723868
    • Vaughan A, Chadee DD, French-Constant R.
      Biochemical monitoring of organophosphorus and carbamate insecticide resistance in Aedes aegypti mosquitoes from Trinidad.
      Med Vet Entomol., 1998 July : 12(3)318-21.
      https://www.ncbi.nlm.nih.gov/pubmed/9737606
    • Vaughan A, Rocheleau T, ffrench-Constant R.
      Site-directed mutagenesis of an acetylcholinesterase gene from the yellow fever mosquito Aedes aegypti confers insecticide insensitivity.
      Exp Parasitol., 1997 Nov. : 87(3)237-44
      https://www.ncbi.nlm.nih.gov/pubmed/9371089
    • DeSilva D, Hemingway J, Ranson H, Vaughan A.
      Resistance to insecticides in insect vectors of disease: est alpha 3, a novel amplified esterase associated with amplified est beta 1 from insecticide resistant strains of the mosquito Culex quinquefasciatus.
      Exp Parasitol., 1997 Nov. : 87(3)253-9.
      https://www.ncbi.nlm.nih.gov/pubmed/9371091
    • Vaughan A, Hawkes N, Hemingway J.
      Co-amplification explains linkage disequilibrium of two mosquito esterase genes in insecticide-resistant Culex quinquefasciatus.
      Biochem J., 1997 July 15 : 325(Pt 2)359-65 PMCID:PMC1218568
      https://www.ncbi.nlm.nih.gov/pubmed/9230114
    • Ranson H, Cornel AJ, Fournier D, Vaughan A, Collins FH, Hemingway J.
      Cloning and localization of a glutathione S-transferase class I gene from Anopheles gambiae.
      J Biol Chem., 1997 Feb. 28 : 272(9)5464-8.
      https://www.ncbi.nlm.nih.gov/pubmed/9038148
    • Karunaratne SH, Hemingway J, Jayawardena KG, Dassanayaka V, Vaughan A.
      Kinetic and molecular differences in the amplified and non-amplified esterases from insecticide-resistant and susceptible Culex quinquefasciatus mosquitoes.
      J Biol Chem., 1995 Dec. 29 : 270(52)31124-8.
      https://www.ncbi.nlm.nih.gov/pubmed/8537374
    • Vaughan A, Hemingway J.
      Mosquito carboxylesterase Est alpha 2(1) (A2). Cloning and sequence of the full-length cDNA for a major insecticide resistance gene worldwide in the mosquito Culex quinquefasciatus.
      J Biol Chem., 1995 July 14 : 270(28)17044-9.
      https://www.ncbi.nlm.nih.gov/pubmed/7622525
    • Vaughan A, Rodriguez M, Hemingway J.
      The independent gene amplification of electrophoretically indistinguishable B esterases from the insecticide-resistant mosquito Culex quinquefasciatus.
      Biochem J., 1995 Jan. 15 : 305(Pt 2)651-8.
      https://www.ncbi.nlm.nih.gov/pubmed/7530448
    • Vaughan A, Ketterman AJ, Hemingway J.
      Comparisons of nucleic acid sequences of esterases from resistant and susceptible strains of Culex quinquefasciatus.
      Biochem Soc Trans., 1993 Nov. : 21(4)481S
      https://www.ncbi.nlm.nih.gov/pubmed/8132048
    • Sumner IG, Vaughan A, Eisenthal R, Pickersgill RW, Owen AJ, Goodenough PW.
      Kinetic analysis of papaya proteinase omega.
      Biochim Biophys Acta., 1993 Aug. 7 : 1164(3)243-51.
      https://www.ncbi.nlm.nih.gov/pubmed/8393709
    • Vize PD, Vaughan A, Krieg P.
      Expression of the N-myc proto-oncogene during the early development of Xenopus laevis.
      Development., 1990 Nov. : 110(3)885-96.
      https://www.ncbi.nlm.nih.gov/pubmed/2088727

    Book Chapters

    • Vaughan AM, Kappe SHI.
      Malaria Parasite Liver Infection and Exoerythrocytic Biology.
      Cold Spring Harb Perspect Med., 2017 June 1 : 7(6)pii: a025486. PMCID:PMC5453383
      https://www.ncbi.nlm.nih.gov/pubmed/28242785
    • Sack BK, Miller JL, Vaughan AM, Kappe SH.
      Measurement of Antibody-Mediated Reduction of Plasmodium yoelii Liver Burden by Bioluminescent Imaging.
      Methods Mol Biol., 2015 : 132569-80
      https://www.ncbi.nlm.nih.gov/pubmed/26450380
    • Vaughan AM, Kappe SH.
      Vaccination using radiation- or genetically attenuated live sporozoites.
      Methods Mol Biol., 2013 : 923549-66
      https://www.ncbi.nlm.nih.gov/pubmed/22990804
    • Vaughan AM.
      Fatty Acid Synthesis in the Apicoplast.
      Encyclopedia of Malaria., 2013

    Published Books, Videos, Software, etc.

    • Editor: Vaughan AM.
      Malaria Vaccines.
      Methods Mol Biol., 2015 : 1325v-vi.
      https://www.ncbi.nlm.nih.gov/pubmed/26730424

    Other Publications

    • Itsara LS, Zhou Y, Do J, Grieser AM, Vaughan AM, Ghosh AK.
      The Development of Whole Sporozoite Vaccines for Plasmodium falciparum Malaria.
      Front Immunol., 2018 Dec. 11 : 92748. Review. PMCID:PMC6297750
      https://www.ncbi.nlm.nih.gov/pubmed/30619241
    • Vaughan AM, Kappe SHI.
      Genetically attenuated malaria parasites as vaccines.
      Expert Rev Vaccines., 2017 Aug. : 16(8)765-767
      https://www.ncbi.nlm.nih.gov/pubmed/28612631
    • Vaughan AM, Kappe SH.
      Malaria vaccine development: persistent challenges.
      Curr Opin Immunol., 2012 June : 24(3)324-31. Review.
      https://www.ncbi.nlm.nih.gov/pubmed/22405559
    • Butler NS, Vaughan AM, Harty JT, Kappe SH.
      Whole parasite vaccination approaches for prevention of malaria infection.
      Trends Immunol., 2012 May : 33(5)247-54. Review.
      https://www.ncbi.nlm.nih.gov/pubmed/22405559
    • Kappe SH, Vaughan AM, Boddey JA, Cowman AF.
      That was then but this is now: malaria research in the time of an eradication agenda.
      Science., 2010 May 14 : 328(5900)862-6. Review.
      https://www.ncbi.nlm.nih.gov/pubmed/20466924
    • Vaughan AM, Wang R, Kappe SH.
      Genetically engineered, attenuated whole-cell vaccine approaches for malaria.
      Hum Vaccin., 2010 Jan. : 6(1)107-13. Review. PMCID:PMC3641786
      https://www.ncbi.nlm.nih.gov/pubmed/19838068
    • Tarun AS, Vaughan AM, Kappe SH.
      Redefining the role of de novo fatty acid synthesis in Plasmodium parasites.
      Trends Parasitol., 2009 Dec. : 25(12)545-50.
      https://www.ncbi.nlm.nih.gov/pubmed/19819758
    • Aly AS, Vaughan AM, Kappe SH.
      Malaria parasite development in the mosquito and infection of the mammalian host.
      Annu Rev Microbiol., 2009 : 63195-221. Review. PMCID:PMC2841446
      https://www.ncbi.nlm.nih.gov/pubmed/19575563
    • Vaughan AM, Aly AS, Kappe SH.
      Malaria parasite pre-erythrocytic stage infection: gliding and hiding.
      Cell Host Microbe., 2008 Sept. 11 : 4(3)209-18. Review. PMCID:PMC2610487
      https://www.ncbi.nlm.nih.gov/pubmed/18779047
    • Oram JF, Vaughan AM.
      ATP-Binding cassette cholesterol transporters and cardiovascular disease.
      Circ Res., 2006 Nov. 10 : 99(10)1031-43. Review.
      https://www.ncbi.nlm.nih.gov/pubmed/17095732
    • Oram JF, Vaughan AM.
      ABCA1-mediated transport of cellular cholesterol and phospholipids to HDL apolipoproteins.
      Curr Opin Lipidol., 2000 June : 11(3)253-60. Review.
      https://www.ncbi.nlm.nih.gov/pubmed/10882340
    • ffrench-Constant RH, Pittendrigh B, Vaughan A, Anthony N.
      Why are there so few resistance-associated mutations in insecticide target genes?
      Philos Trans R Soc Lond B Biol Sci., 1998 Oct. 29 : 353(1376)1685-93. Review. PMCID:PMC1692388
      https://www.ncbi.nlm.nih.gov/pubmed/10021768

  • Presentations Title Event Location Date
    Using experimental genetic crosses to understand malaria parasite evolution and fitness. Center for Global Infectious Disease Research, Global Infectious Disease Seminar Series Seattle Children’s Institute, Seattle, WA 2019
    Killing me softly: the creation of effective malaria vaccines by genetic attenuation of the parasite. Texas Biomedical Research Institute Seminar Series San Antonio, TX 2018
    New tools for the generation of genetically attenuated Plasmodium falciparum for vaccine development. Joint International Tropical Medicine Meeting, “Tropical Medicine 4.0: Effective Collaboration for an Impact on Global Health” Bangkok, Thailand 2017
    Taking advantage of Plasmodium falciparum genetic recombination to study drug resistance. Johns Hopkins Malaria Research Institute Seminar Series Baltimore, MD 2016
    What can we learn from Plasmodium falciparum sex? Center for Infectious Disease Research Global Health Seminar Series Seattle, WA 2016
    Tracking malaria drug resistance traits in humanized mice. Gordon Research Conference on Drug Resistance University of New England, Biddeford, ME 2016
    Human liver chimeric mice for experimental Plasmodium falciparum crosses. Joint International Tropical Medicine Meeting, “Tropical Diseases Post 2015: New Threats or Towards Sustainable Success” Bangkok, Thailand 2015
    Human malaria liver stage development in liver-chimeric mice. Royal Society of Tropical Medicine and Hygiene Biennial Meeting, “Discovery and Delivery of New Paradigms for Global Health” University of Warwick, UK 2012
    Plasmodium vivax liver stage development and hypnozoite formation in a human liver chimeric mouse model. Joint International Tropical Medicine Meeting, “Emergence of Tropical Diseases: Trans-disciplinary strategies” Bangkok, Thailand 2012
    Plasmodium fatty acid metabolism. Seattle Malaria Group Meeting Seattle, WA 2010
    ABCA1 and ABCG1 contribute to the removal of excess cholesterol from loaded macrophages. Gordon Research Conference on Lipoprotein Metabolism Mount Holyoke College, MA 2006
  • Grant Title Grantor Amount Award Date
    Mechanisms of nutrient acquisition by malaria parasite mosquito stages - R21AI146391 (Vaughan) NIH $275,000 July 1, 2019 - June 30, 2021
    A chemical proteomics survey of Plasmodium gametocyte development- R21AI133388 A1 (Grundner) NIH $150,000 April 1, 2018 - March 31, 2020
    Harnessing the power of experimental genetic crosses and systems genetics to probe drug resistance in malaria - P01AI27338 (Ferdig) Notre Dame/NIH $281,920 Aug. 1, 2017 - July 31, 2022
    A systems genetics approach to determine factors regulating Plasmodium falciparum sporozoite infectivity- R21AI133369 (Vaughan) NIH $125,000 July 1, 2017 - June 30, 2019
    VTEU Protocol Development, Task 1 Protocol 14-0088- HHSN272201300019I-CIDR- Protocol VTEU #14-0088 (Jackson) Kaiser Foundation/NIAID $369,129 July 1, 2017 - Feb. 29, 2020
    Engineering of genetically attenuated pre-erythrocytic Plasmodium parasites for cross-stage protective immunity - R01AI125706 (Kappe) NIH $250,000 July 1, 2016 - June 30, 2021

Overview

Research Description

The mission of the Vaughan Lab ultimately is to alleviate the suffering of those affected by the disease malaria, which kills upwards of 400,000 people, mostly young children, every year. We study both the human malaria parasites Plasmodium falciparum and Plasmodium vivax as well as the rodent malaria models Plasmodium yoelii and Plasmodium berghei. Read about the Vaughan Lab.

Research Focus Area

Biotechnology, Drug Resistance, Genetic Engineering, Genetics, Global Health, Host-Pathogen Interaction, Immunology, Infectious Disease, Malaria, Systems Biology