Richard G. James, PhD

Richard G. James, PhD

Children's Title: Principal Investigator, Seattle Children's Research Institute

Academic Title: Assistant Professor, Department of Pediatrics, University of Washington

Research Center: Center for Immunity and Immunotherapies

Making a Difference

  • New Insights on Craniosynostosis

    Seattle Children’s researchers found that the mild to moderate developmental delays that can accompany the most common type of craniosynostosis can be overcome. ... cont.


Medical/Professional School

Harvard University, Cambridge
University of Michigan, Ann Arbor

About My Work

Awards and Honors

Award Name Award Description Awarded By Award Date
K99 NHLBI 2010 - 2012
Pre-doctoral Fellowship Howard Hughes Medical Institute 1999 - 2004
Undergraduate Training Grant National Science Foundation 1997 - 1998


  • James RG, Bosch KA, Kulikauskas RM, Yang PT, Robin NC, Toroni RA, Biechele TL, Berndt JD, von Haller PD, Eng JK, Wolf-Yadlin A, Chien AJ, Moon RT
    Protein Kinase PKN1 Represses Wnt/-Catenin Signaling in Human Melanoma Cells.
    24114839 The Journal of biological chemistry , 2013 Nov. 29 : 288(48)34658-70 PMCID: PMC3843078
  • Moore KE, Carlson SM, Camp ND, Cheung P, James RG, Chua KF, Wolf-Yadlin A, Gozani O
    A general molecular affinity strategy for global detection and proteomic analysis of lysine methylation.
    23583077 Molecular cell , 2013 May 9 : 50(3)444-56 PMCID: PMC3660009
  • Fleming BM, Yelin R, James RG, Schultheiss TM
    A role for Vg1/Nodal signaling in specification of the intermediate mesoderm.
    23533180 Development (Cambridge, England) , 2013 Apr, : 140(8)1819-29 PMCID: PMC3621495
  • Camp ND, James RG, Dawson DW, Yan F, Davison JM, Houck SA, Tang X, Zheng N, Major MB, Moon RT
    Wilms tumor gene on X chromosome (WTX) inhibits degradation of NRF2 protein through competitive binding to KEAP1 protein.
    22215675 The Journal of biological chemistry , 2012 Feb. 24 : 287(9)6539-50 PMCID: PMC3307315
  • Biechele TL, Kulikauskas RM, Toroni RA, Lucero OM, Swift RD, James RG, Robin NC, Dawson DW, Moon RT, Chien AJ
    Wnt/-catenin signaling and AXIN1 regulate apoptosis triggered by inhibition of the mutant kinase BRAFV600E in human melanoma.
    22234612 Science signaling , 2012 Jan. 10 : 5(206)ra3 PMCID: PMC3297477
  • James RG, Davidson KC, Bosch KA, Biechele TL, Robin NC, Taylor RJ, Major MB, Camp ND, Fowler K, Martins TJ, Moon RT
    WIKI4, a novel inhibitor of tankyrase and Wnt/ß-catenin signaling.
    23227175 PloS one , 2012 : 7(12)e50457 PMCID: PMC3515623
  • Kamei CN, Kempf H, Yelin R, Daoud G, James RG, Lassar AB, Tabin CJ, Schultheiss TM
    Promotion of avian endothelial cell differentiation by GATA transcription factors.
    21354132 Developmental biology , 2011 May 1 : 353(1)29-37 PMCID: PMC3165022
  • Cimetta E, Cannizzaro C, James R, Biechele T, Moon RT, Elvassore N, Vunjak-Novakovic G
    Microfluidic device generating stable concentration gradients for long term cell culture: application to Wnt3a regulation of -catenin signaling.
    20936235 Lab on a chip , 2010 Dec. 7 : 10(23)3277-83
  • James RG, Biechele TL, Conrad WH, Camp ND, Fass DM, Major MB, Sommer K, Yi X, Roberts BS, Cleary MA, Arthur WT, MacCoss M, Rawlings DJ, Haggarty SJ, Moon RT
    Bruton's tyrosine kinase revealed as a negative regulator of Wnt-beta-catenin signaling.
    19471023 Science signaling , 2009 May 26 : 2(72)ra25
  • James RG, Conrad WH, Moon RT
    Beta-catenin-independent Wnt pathways: signals, core proteins, and effectors.
    19099251 Methods in molecular biology (Clifton, N.J.) , 2008 : 468131-44
  • James RG, Kamei CN, Wang Q, Jiang R, Schultheiss TM
    Odd-skipped related 1 is required for development of the metanephric kidney and regulates formation and differentiation of kidney precursor cells.
    16790474 Development (Cambridge, England) , 2006 Aug. : 133(15)2995-3004
  • James RG, Schultheiss TM
    Bmp signaling promotes intermediate mesoderm gene expression in a dose-dependent, cell-autonomous and translation-dependent manner.
    16243309 Developmental biology , 2005 Dec. 1 : 288(1)113-25
  • Wilm B, James RG, Schultheiss TM, Hogan BL
    The forkhead genes, Foxc1 and Foxc2, regulate paraxial versus intermediate mesoderm cell fate.
    15196959 Developmental biology , 2004 July 1 : 271(1)176-89
  • Deakin S, Leviev I, Guernier S, James RW
    Simvastatin modulates expression of the PON1 gene and increases serum paraoxonase: a role for sterol regulatory element-binding protein-2.
    14500290 Arteriosclerosis, thrombosis, and vascular biology , 2003 Nov. 1 : 23(11)2083-9
  • James RG, Schultheiss TM
    Patterning of the avian intermediate mesoderm by lateral plate and axial tissues.
    12490201 Developmental biology , 2003 Jan. 1 : 253(1)109-24


Presentations Title Event Location Date
PKN1 inhibits Wnt-b-catenin signaling and is required for growth in melanoma American Society for Mass Spectrometry National Conference Vancouver, BC Canada May 20, 2012
Integration of phosphoproteomic and siRNA screens of the Wnt/beta-catenin pathway unearth novel drug targets in melanoma Annual Converence - American Society for Mass Spectrometry Vancouver, BC May 20, 2012

Research Funding

Grant Title Grantor Amount Award Date
In vivo role of Btk-mediated inhibition of Wnt/beta-catenin signaling during hematopoiesis NIH - NHLBI Aug. 15, 2010 - May 30, 2015