Profile

Thor A. Wagner, MD

Thor A. Wagner, MD

Infectious Disease

On staff since July 2008

Academic Title: Assistant Professor

Research Center: Center for Global Infectious Disease Research

"My research is inspired by patients that I meet and directed at the problems that they face. There are exciting opportunities to adapt current biotechnology to help our patients' needs, and it's great motivation to know that the research we do has the potential to directly benefit our patients."

Thor Wagner, MD: Assistant Professor of Pediatric Infectious Diseases. Dr. Wagner's research is focused on pediatric HIV infection, which accounts for 15% of all HIV deaths.

My primary interest is understanding chronic HIV infection during antiretroviral therapy. Specifically, why doesn't antiretroviral therapy eradicate HIV infection? Is there ongoing viral replication? Is there proliferation of cells with viable proviral HIV? Can we identify the remaining infected cells? Is immune tolerance to HIV a barrier to curing HIV? Answers to these questions should help design new treatment strategies more likely to cure HIV.
My other research interest is improving infectious disease diagnostics in low resource settings. Specifically, I am working to develop a point-of-care diagnostic test for infant HIV. Currently there is no simple test to diagnose infants with HIV, and 50% of HIV-infected children die before they can be diagnosed. To accomplish this we are utilizing state-of-the-art monoclonal antibody screening technology to optimize the sensitivity of immunoassays to detect HIV antigens.

Overview

Board Certification(s)

Pediatrics
Pediatric Infectious Diseases

Medical/Professional School

Temple University School of Medicine, Philadelphia

Residency

Pediatrics, Children's Hospital of New York, CHN-517, New York

Fellowship

Infectious Disease, Seattle Children's Hospital, Seattle

Clinical Interests

General pediatric infectious diseases, pediatric HIV Mechanism of persistent HIV infection despite effective treatment, improving HIV diagnostics for the low resource settings

Research Description

My research is focused on pediatric HIV infection, which accounts for 15percent of all HIV deaths.

My primary interest is understanding chronic HIV infection during antiretroviral therapy. Specifically, why doesn't antiretroviral therapy eradicate HIV infection? Is there ongoing viral replication? Is there proliferation of cells with viable proviral HIV? Can we identify the remaining infected cells? Is immune tolerance to HIV a barrier to curing HIV? Answers to these questions should help design new treatment strategies more likely to cure HIV.

My other research interest is improving infectious disease diagnostics in low resource settings. Specifically, I am working to develop a point-of-care diagnostic test for infant HIV. Currently there is no simple test to diagnose infants with HIV, and 50percent of HIV-infected children die before they can be diagnosed. To accomplish this we are utilizing state-of-the-art monoclonal antibody screening technology to optimize the sensitivity of immunoassays to detect HIV antigens.

Research Focus Area

Infectious Disease

Awards and Honors

Award Name Award Description Awarded By Award Date
 Young Scientist Scholarship - XVIII HIV International Drug Resistance Workshop XVIII HIV International Drug Resistance Workshop Jan. 1, 2009
Postdoctoral Fellowship in Tropical Infectious Disease Burroughs Wellcome Fund, American Society of Tropical Medicine and Hygiene 2008 - 2010
Young Investigator Award 15th Conf. on Retroviruses and Opportunistic Infections 2008
Department of Pediatrics Fellows' Research Day Award Seattle Children's Research Institute 2007

Publications

  • Wagner TA, McLaughlin S, Garg K, Cheung CY, Larsen BB, Styrchak S, Huang HC, Edlefsen PT, Mullins JI, Frenkel LM
    Proliferation of cells with HIV integrated into cancer genes contributes to persistent infection.
    Science (New York, N.Y.) , 2014 July
  • Wagner TA, McKernan JL, Tobin NH, Tapia KA, Mullins JI, Frenkel LM
    An increasing proportion of monotypic HIV-1 DNA sequences during antiretroviral treatment suggests proliferation of HIV-infected cells.
    Journal of virology , 2013 Feb. : 87(3)1770-8
  • Wagner TA, Lin CH, Tobin NH, Côté HC, Sloan DD, Jerome KR, Frenkel LM
    Quantification of mitochondrial toxicity in HIV-infected individuals by quantitative PCR compared to flow cytometry.
    Cytometry. Part B, Clinical cytometry , 2013 Jan. : 84(1)55-8
  • Beck IA, Jang M, McKernan-Mullin J, Bull M, Wagner T, Huang S, Song LY, Nachman S, Krogstad P, Eshleman SH, Wiznia A, Frenkel LM
    Monitoring of HIV type 1 DNA load and drug resistance in peripheral blood mononuclear cells during suppressive antiretroviral therapy does not predict virologic failure.
    AIDS research and human retroviruses , 2012 Aug. : 28(8)780-8
  • Wagner TA, Frenkel LM
    Yet Another Reason to Treat HIV Infection.
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America , 2012 Apr, : 54(7)1010-2
  • Thor A. Wagner, MD
    Emerging Biomarkers for the Diagnosis of Severe Neonatal Infections Applicable to Low Resource Settings
    Journal of Global Health , 2011 : 1(2)208-221
  • Thor A. Wagner, MD
    Epidemiology and aetiology of parasitic maternal infections in low- and middleincome countries
    Journal of Global Health , 2011 : 1(2)187-198
  • Thor A. Wagner, MD
    Epidemiology and aetiology of maternal bacterial and viral infections in low and middle income countries
    Journal of Global Health , 2011 : 1(2)169-185
  • Thor A. Wagner, MD
    A global health opportunity: the potential of multiplexed diagnostics in low resource settings
    Journal of Global Health , 2011 : 1(2)136-139
  • Wagner TA, Frenkel LM
    Antiretroviral treatment for HIV-infected infants exposed to nevirapine.
    JAMA : the journal of the American Medical Association , 2010 Dec. : 304(23)2589; author reply 2589-90
  • Quan PL, Wagner TA, Briese T, Torgerson TR, Hornig M, Tashmukhamedova A, Firth C, Palacios G, Baisre-De-Leon A, Paddock CD, Hutchison SK, Egholm M, Zaki SR, Goldman JE, Ochs HD, Lipkin WI
    Astrovirus encephalitis in boy with X-linked agammaglobulinemia.
    Emerging infectious diseases , 2010 June : 16(6)918-25
  • Wagner TA, Kress CM, Beck I, Techapornroong M, Wittayapraparat P, Tansuphasawasdikul S, Jourdain G, Ngo-Giang-Huong N, Lallemant M, Frenkel LM
    Detection of HIV-1 drug resistance in women following administration of a single dose of nevirapine: comparison of plasma RNA to cellular DNA by consensus sequencing and by oligonucleotide ligation assay.
    Journal of clinical microbiology , 2010 May : 48(5)1555-61
  • Jourdain G, Wagner TA, Ngo-Giang-Huong N, Sirirungsi W, Klinbuayaem V, Fregonese F, Nantasen I, Techapornroong M, Halue G, Nilmanat A, Wittayapraparat P, Chalermpolprapa V, Pathipvanich P, Yuthavisuthi P, Frenkel LM, Lallemant M, Program for HIV Prevention and Treatment (PHPT) Study Group
    Association between detection of HIV-1 DNA resistance mutations by a sensitive assay at initiation of antiretroviral therapy and virologic failure.
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America , 2010 May : 50(10)1397-404
  • Wagner TA, Kress CM, Beck I, Techapornroong M, Wittayapraparat P, Tansuphasawasdikul S, Jourdain G, Ngo-Giang-Huong N, Lallemant M, Frenkel LM
    Detection of HIV-1 drug resistance in women following administration of a single dose of nevirapine: comparison of plasma RNA to cellular DNA by consensus sequencing and by oligonucleotide ligation assay.
    Journal of clinical microbiology , 2010 May : 1555-61
  • Lin CH, Sloan DD, Dang CH, Wagner T, Cabrera AJ, Tobin NH, Frenkel LM, Jerome KR
    Assessment of mitochondrial toxicity by analysis of mitochondrial protein expression in mononuclear cells.
    Cytometry. Part B, Clinical cytometry , 2009 May : 76(3)181-90
  • Wagner TA, Tobin NH, McKernan JL, Xu M, Melvin AJ, Mohan KM, Learn GH, Mullins JI, Frenkel LM
    Increased mutations in Env and Pol suggest greater HIV-1 replication in sputum-derived viruses compared with blood-derived viruses.
    AIDS (London, England) , 2009 May : 23(8)923-8
  • Wagner TA, Frenkel LM
    Potential limitation of CCR5 antagonists: drug resistance more often linked to CXCR4-utilizing than to CCR5-utilizing HIV-1.
    AIDS (London, England) , 2008 Nov. : 22(17)2393-5
  • Wagner T, Burns JL
    Anti-inflammatory properties of macrolides.
    The Pediatric infectious disease journal , 2007 Jan. : 26(1)75-6
  • Thor A. Wagner, MD
    Clinical Significance of HIV-1 Drug Resistance Mutations
    J. of Lab. Medicine , 2006 : 37: 554-561
  • Wagner T, Soong G, Sokol S, Saiman L, Prince A
    Effects of azithromycin on clinical isolates of Pseudomonas aeruginosa from cystic fibrosis patients.
    Chest , 2005 Aug. : 128(2)912-9

Research Funding

Grant Title Grantor Amount Award Date
"Mechanisms of Formation and Persistence of Active HIV Reservoirs" - R01 AI111806 (PI: Mullins) NIH $10,720 subcontract annual direct costs March 15, 2014 - Feb. 28, 2019
Systematic High-Throughput Screening of B-cell Repertoires to Identify Antibodies Specific for HIV-Infected Cells Bill and Melinda Gates Foundation $ Nov. 1, 2011 - April 30, 2013
HIV Persistence Despite Therapy due to Proliferation of Infected Cells Fraternal Order of Eagles Oct. 1, 2009 - Sept. 30, 2014
Novel microcapillary immunoassay methodology to enable point-of-care infant HIV-1 diagnosis Seattle Children’s Hospital Jan. 1, 2009
Persistent HIV-1 Replication in the Respiratory Tract Despite Antiretroviral Treatment NIH-NIAID Sept. 1, 2008 - Nov. 30, 2013
Improving Pediatric HIV-1 Diagnostics in an Effort to Avoid Antiretroviral Treatment Failure in Tamilnadu, India Burroughs Wellcome Fund/American Society of Tropical Medicine and Hygiene July 1, 2008 - June 30, 2014