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Center for Developmental Therapeutics Research Cores

The Center for Developmental Therapeutics is developing resources to support the greater research community. Our research cores include training, technical and intellectual assistance on projects and access to potential collaborators.

All members of the research community are encouraged to make use of these resources. Comments or suggestions are welcome.

We currently have three cores available to the community.

High-Throughput Data Analysis Core

The mission of Seattle Children’s High-Throughput Data Analysis Core (HAC) is to enable biomedical discoveries by translating data to knowledge. We provide the research community with analysis and integration expertise as well as data acquisition (proteomics) services. Our services are available for individual project sections and phases or for the full cycle of your project including funding applications.

To learn more about the HAC, please contact Gregory Yandl, business development specialist.

Next Generation Sequencing Core

The Sequencing Core provides access to next generation sequencing technology based in the laboratory of Sihoun Hahn, MD, PhD.

The Hahn lab has an Illumina Genome Analyzer IIx (GAIIx), a next-generation sequencer that performs massively parallel sequencing-by-synthesis. The GAIIx is capable of generating 80 billion bases of DNA sequence per run and is ideal for targeted capture, ChIP-seq, smallRNA-seq and small genome re-sequencing.

Potential applications for the GAIIx include DNA sequencing, gene regulation analysis, sequencing-based transcriptome analysis, SNP discovery and structural variation analysis, cytogenetic analysis, DNA–protein interaction analysis (ChIP-Seq) and sequencing-based methylation analysis.

The core is able to offer access to this instrument for small scale sequencing project. The core will provide sequencing and standard data analysis, which includes converting raw image data to base calls, providing quality scores at each base and aligning sequences to a reference genome. Sample prep, data interpretation and validation might be available for some projects, but will need to be discussed with the Hahn lab.

To learn more about the Sequencing Core, please contact Valeria Vasta, PhD, technical director of the Hahn lab.

Luminex Core

Our Luminex Core provides access to a Luminex 200 instrument and expertise in bioassays development and analysis.  The primary users are Seattle Children’s investigators, but we welcome inquiries from external non-profit and for-profit groups.

The Luminex system is a flexible analyzer based on the principles of flow cytometry. Capabilities include:

  • Ability to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small sample volumes
  • Delivering fast and cost-effective bioassay results on many assay formats, such as nucleic acid assays, receptor-ligand assays, immunoassays and enzymatic assays

The instrument runs MasterPlex QT v3.0 and MasterPlex CT v1.0 software, which is also convenient for data transfer and customized assay and data protocols.

User fees have been established for use of the facility. To learn more and gain access, please send an inquiry; internal users may also visit the CDT Luminex Core SharePoint.

Core Personnel
Scientific/Technical Inquiries:Marta Bull, PhD 
Financial/Operational Inquiries:Andrew Lowe, MPA 

All Research Cores

Seattle Children’s Research Institute houses additional core laboratories and services. See all Seattle Children's Research Cores.

 

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