Protein Expression

  • BAFF receptor
  • BTK  
  • CD18
  • CD40
  • CD40 ligand
  • FOXP3  
  • ICOS
  • ICOS ligand
  • Interleukin-7 receptor alpha (CD127)
  • SAP (SH2D1A)
  • SPINK5
  • WASP  
  • XIAP (BIRC4)

Functional Analysis

  • CD40 binding to CD40 ligand (done routinely with CD40L protein expression)
  • IκBα phosphorylation/degradation assay (screening for EDA-ID)
  • Lymphocyte apoptosis, Fas-mediated (screening test for ALPS)
  • Neutrophil oxidative burst assay (screening test for chronic granulomatous disease)   
  • NK cell/cytotoxic T-cell degranulation assay (CD107)
  • STAT1 phosphorylation in response to interferon gamma (screening test for interferon-gamma receptor deficiency)
  • STAT3 phosphorylation in response to IL-6 (HIES screening)
  • STAT4 phosphorylation in response to interferon alpha and IL-12 (screening test for IL-12 receptor deficiency and Tyk2 deficiency)
  • STAT5B phosphorylation in response to IL-2 and IL-7 (screening test for common gamma chain, JAK3 and IL-7 receptor deficiency)

Immunophenotyping

Bacteriophage Immunization

  • For information or to arrange bacteriophage immunization to evaluate immune responses, call 206-884-7397.