Richard James, PhD, is a principal investigator at Seattle Children’s Research Institute’s Center for Immunity and Immunotherapies and an assistant professor at the University of Washington. He received his PhD from Harvard University and completed a postdoctoral fellowship at the University of Washington.
B-Cell Biology and Immunological Disease
The James Lab is interested in uncovering biochemical mechanisms that contribute to drug resistance and to immunological disease. To do so, we investigate many features of protein behavior, including their impact on differentiation, their interactions with other molecules and whether mutation regulates these processes. The current focuses of the lab include:
- Leveraging our ability to genetically engineer primary human B cells to create protein-producing cell therapies and to understand the genetic events that promote differentiation into long-lived antibody secreting cells
- Determining which signaling events are required for resistance to targeted therapies and developing therapeutic strategies to block these events
- Mapping the effects of each possible variant of genes involved in primary immune deficiency and autoimmunity by combining multiplexed genome editing, signaling assays and high-throughput DNA sequencing. In the course of these studies, we hope to create look-up tables that can be used by clinicians to help interpret sequencing data from patients.
Our work was featured in Seattle Children’s Research Institute’s On the Pulse blog and in the 2015 Academic Annual Report.