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Pathology Integrative Resource Core (PIRC)

PIRC Services and Fees

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New Study Setup Administrative Fees Seattle Children's fee External fee Unit 
Level 1$8.00$11.84Per study
Level 2$93.00$137.64Per study
Level 3$238.00$352.24Per study


Histology Services Seattle Children's fee External fee Unit 
Paraffin processing and sectioning    
Trim to cassette$2.00$2.96Each
Paraffin process/embed$5.00$7.40Each
Cut unstained, first slide$4.00$5.92Each
Cut unstained, additional slides$2.00$2.96Each
Thick cut ribbon$8.00$11.84Each
Processing and sectioning    
Snap frozen in cryovial or cassette$10.00$14.80Each
Embed, cryosection, first slide$10.00$14.80Each
Embed, cryosection, additional slides$2.50$3.70     Each
Fresh tissue in PBS or RPMI$10.00$14.80Each


Staining Seattle Children's fee External fee Unit 
H&E$2.50$3.70Per slide
AFB/Fe/PAS/PAS-D/Alcian blue/LFB/GMS/Reticulin/WG$8.50$12.58Per slide
MT/VVG/Congo/Sirius/Steiner/colloidal Fe/ORO/Gram Pentachrome$12.50$18.50Per slide
In situ hybridization (you provide the probe)$40.00$59.20Per slide
In situ hybridization (we provide the probe)$25.00$37.00Per slide
 Immunostaining    
Established immunostain$25.00$37.00Per slide
Antibody workup$225.00$333.00Per antibody
Dual immunostain$60.00$88.80Per slide
Immunofluorescence$45.00$66.60Per slide
Dual immunofluorescence$57.00$84.36Per slide
Triple immunofluorescence$70.00$103.60Per slide


 Electron microscopy Seattle Children's fee External fee Unit 
EM tissue processing/embed$20.00$29.60Per block
EM sectioning and scoping $40.00$59.20Hourly


Tissue Microarrays Seattle Children's fee External fee Unit 
Consultation and setup (tissue provided by researcher)$150.00$222.00Each
Consultation and setup (tissue provided by core)$450.00$666.00Each
TMA construction (tissue core placement in TMA block)$3.75$5.55Per core
Unstained slide from TMA block$7.40$10.95Per slide


Molecular Studies Seattle Children's fee External fee Unit 
DNA isolation from fresh or frozen tissue sample$25.00$37.00Each
DNA isolation from blood/saliva$20.00$29.60Each
RNA isolation/QC check from fresh or frozen tissue sample$40.00$59.20Each


Shipping Seattle Children's fee External fee Unit 
Frozen shipping$32.40$47.95Each
Room-temperature shipping$16.75$24.79Each


Specimen Storage Seattle Children's fee External fee Unit 
Freezer storage (-80°C) (additional tech fee for redoing inventory)$12.00$17.76Per box/year
Liquid nitrogen storage$75.00$111.00Per box/year


Additional Services Seattle Children's fee External fee Unit 
Autopsy for research tissue collection$1,000.00$1,480.00Each
OR pickup$18.00$29.60Each
Off hours sample pickupVariesVariesEach
Liquid specimen aliquotting (1–5)$6.00$8.88Each
Liquid specimen aliquotting (6–10)$8.00$11.84Each
Liquid specimen processing (spin, aliquot) (1–5)$9.00$13.32Each
Liquid specimen processing (spin, aliquot) (6–10)$12.00$17.76Each
Pregnancy test$7.50$11.10     Each
Processing of urine and blood$15.00$22.20Each
Bone marrow processing with Ficoll$100.00$148.00Each
Lysosomal Acid Lipase (LAL)$46.00$68.08Each
PBMC isolation$35.00$51.80Each
Pulling slides and blocks$100.00$148.00Hourly
Photography support$23.78$35.19Hourly
Pathologist, hourly fee to cover other services$100.00$148.00Hourly
Technician, hourly fee to cover other services$23.78$35.19Hourly

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