The Flow Cytometry Core supports faculty and students at Seattle Children's Research Institute as well as investigators at other academic institutions and private companies.
The core provides access to state-of-the-art cell sorters, analyzers and workstations; training and technical support of investigators; assistance with experimental design, data analysis and interpretation; and assistance in developing novel multicolor flow cytometric techniques.
In addition to reading about the
available in our lab, you can view links to helpful information about
that may be helpful as you set up and run your experiments.
The BD FACSCalibur is a four-color, six-parameter instrument equipped with an air-cooled, 488-nm argon-ion laser and a 633-nm red-diode laser.
The BD LSR II is a 15-color, 17-parameter instrument equipped with four solid-state lasers: a 355-nm UV laser, a 488-nm blue laser, a 561-nm green laser and a 640-nm red laser. The LSR II also has a high throughput system (HTS) that acquires samples from multiwell plates.
The BD FACSAria is a nine-color, 11-parameter, high-speed digital bench-top cell sorter equipped with three fiber-optic-linked solid-state lasers (488 nm, 635 nm and 407 nm).
The BD FACSAria #2 is a 15-color, 17-parameter instrument equipped with five lasers: a 355-nm UV laser, a 407-nm violet laser, a 488-nm blue laser, a 561-nm green laser and a 640-nm red laser. The instrument can perform two- and four-way bulk sorting for a variety of tube sizes. The Aria has the automated cell deposition unit (ACDU) for sorting into multiwell plates.
The Aria is also equipped with an aerosol management option (AMO) to control the risk of biohazard exposure by creating a negative laminar-flow pressure in the sort chamber.
Below are links to information on reagents that may be helpful as you set up and run your experiments:
Below are links to information on cytometry tools that may be helpful as you set up and run your experiments:
information for the Flow Cytometry Core.
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