List of Flow Cytometry and Functional Tests Offered

Protein Expression

• BTK  
• CD18
• CD40
• CD40 ligand
• FOXP3  
• ICOS
• Interleukin-7 receptor alpha (CD127)
• SAP (SH2D1A)
• WASP  
• XIAP (BIRC4)

Functional Analysis

• CD40 binding to CD40 ligand (done routinely with CD40L protein expression)
• DOCK8
• HIES Screen
• Iκβα Degradation
• IκBα phosphorylation/degradation assay (screening for EDA-ID)
• IL-10 Receptor
• Lymphocyte apoptosis, Fas-mediated (screening test for ALPS)
• MSMD Screen
• Neutrophil oxidative burst assay (screening test for chronic granulomatous disease)   
• NK cell/cytotoxic T-cell degranulation assay (CD107)
• STAT1 phosphorylation in response to interferon gamma (screening test for interferon-gamma receptor deficiency)
• STAT3 phosphorylation in response to IL-6 (HIES screening)
• STAT4 phosphorylation in response to interferon alpha and IL-12 (screening test for IL-12 receptor deficiency and Tyk2 deficiency)
• STAT5B phosphorylation in response to IL-2 and IL-7 (screening test for common gamma chain, JAK3 and IL-7 receptor deficiency)
• T-cell immunophenotyping (naïve, mature, effector)
• X-SCID Screen

Immunophenotyping

• T-cell, B-cell, and NK-cell quantitation/subset analysis   
• Double-negative αβ T cells (screening test for ALPS)   
• B-cell immunophenotyping (development, memory, switched memory cells)   
• T-cell immunophenotyping (naïve, mature, effector) 
• Regulatory T-cell immunophenotyping 

Bacteriophage Immunization

• For information or to arrange bacteriophage immunization to evaluate immune responses, call 206-884-7397.