Study Uncovers Clues to What Makes Anesthetics Work
Researchers say discovery may reveal new uses of existing medications to reverse anesthesia
Physicians use inhalation anesthetics in a way that is incredibly safe for patients, but very little is known about the intricacies of how these drugs actually work in children and adults. Now, researchers have uncovered what cells respond to anesthesia in an organism known as the C. elegans, according to a new study from the Seattle Children’s Research Institute. C. elegans is a transparent roundworm used often in research. The study, “Optical reversal of halothane-induced immobility in C. elegans,” is published in the December 20, 2011 issue of Current Biology .
“Our findings tell us what cells and channels are important in making the anesthetic work,” said lead author Phil Morgan, MD, researcher at Seattle Children’s Research Institute and University of Washington professor of anesthesiology and pain medicine. “The scientific community has attempted to uncover the secrets of how anesthetics work since the 1860s, and we now have at least part of the answer.” Margaret Sedensky, MD, Seattle Children’s Research Institute and a UW professor of anesthesiology and pain medicine, and Vinod Singaram, graduate student, Case Western Reserve University, are co-lead authors of the study.
The team studied the roundworm after inserting a pigment or protein typically found in the retina of a human eye — called a retinal-dependent rhodopsin channel — into its cells. The proteins in cell membranes act as channels to help movement. Researchers then used a blue light, activating channels in the roundworm that allowed the immediate reversal of anesthetics, and resulting in the roundworm waking up and seemingly swimming off the slide. A video of a roundworm reacting to the blue light, waking up from anesthesia can be found here: http://www.youtube.com/watch?v=FhtFvKlnwxU
The team’s findings won’t immediately translate into a discovery that would be available for humans, cautioned Dr. Morgan, who has been working in this field for some 25 years. “But it tells us what function we have to treat to try to do so,” he said.
“We believe that there is a class of potassium channels in humans that are crucial in this process of how anesthetics work and that they are perhaps the ones that are sensitive to potential anesthesia reversal. There are drugs for blocking these channels and with these same drugs, maybe we can eventually reverse anesthesia.” Potassium channels are found in all living organisms and in most cell types, and they control a wide variety of cell functions.
Anesthesia medications are used in both children and adults, but many are used more often in kids. Dr. Morgan and his colleagues plan to replicate the study in other animal models, starting with a mouse.
Other co-authors for the study include: Benjamin Somerlot, graduate student, Case Western Reserve University; Dr. Scott Falk, University of Pennsylvania Perelman School of Medicine and Dr. Marni Falk, The Children’s Hospital of Philadelphia and University of Pennsylvania Perelman School of Medicine.
The study “Optical reversal of halothane-induced immobility in C. elegans,” in Current Biology can be found here: http://www.cell.com/current-biology/abstract/S0960-9822(11)01203-6
About Seattle Children’s
Seattle Children’s Hospital, Foundation and Research Institute together deliver superior patient care, advance new discoveries and treatments through pediatric research, and raise funds to create better futures for patients. Consistently ranked as one of the top 10 children’s hospitals in the country by U.S. News & World Report, Seattle Children’s Hospital specializes in meeting the unique physical, emotional and developmental needs of children from infancy through young adulthood. Through the collaboration of physicians in nearly 60 pediatric subspecialties, Seattle Children’s Hospital provides inpatient, outpatient, diagnostic, surgical, rehabilitative, behavioral, and emergency and outreach services to families from around the world.
Located in downtown Seattle’s biotech corridor, Seattle Children’s Research Institute is pushing the boundaries of medical research to find cures for pediatric diseases and improve outcomes for children all over the world. Internationally recognized investigators and staff at the research institute are advancing new discoveries in cancer, genetics, immunology, pathology, infectious disease, injury prevention, bioethics and much more.
Seattle Children’s Hospital and Research Foundation and Seattle Children’s Hospital Guild Association work together to gather community support and raise funds for uncompensated care, clinical care and research. The foundation receives nearly 80,000 gifts each year, from lemonade stand proceeds to corporate sponsorships. Seattle Children’s Hospital Guild Association is the largest all-volunteer fundraising network for any hospital in the country, serving as the umbrella organization for 450 groups of people who turn an activity they love into a fundraiser. Support from the foundation and guild association makes it possible for Seattle Children’s care and research teams to improve the health and well-being of all kids.
For more information, visit seattlechildrens.org or follow us on Twitter, Facebook and Instagram.